Inverted Light Microscopy Of Guttae In The Eyebank
Published 2025 - 43rd Congress of the ESCRS
Reference: PP25.02 | Type: Free paper | DOI: 10.82333/33qd-d957
Authors: Andrea Janekova* 1
1Eye Centre Prague,Prague,Czech Republic;Faculty of Medicine in Hradec Kralove, Charles University in Prague and University Hospital Hradec Kralove,Hradec Králové,Czech Republic
Purpose
Since it can be challenging to discriminate between true guttae and artefacts imposing as guttae during eye bank evaluation of donor corneas, we evaluated the morphologic appearance of 'known guttae' in post-mortem eyes with a history of Fuchs endothelial dystrophy (FED).
Setting
Netherlands Institute for Innovative Ocular Surgery, Rotterdam, The Netherlands; Amnitrans EyeBank Rotterdam, The Netherlands
Methods
A total of 22 post-mortem DMEK eyes from 16 FED patients (of which 10 had unilateral DMEK surgery) were examined using inverted light microscopy. Pathologic peripheral host tissue (outside the descemetorhexis) was compared to adjacent central graft tissue in the same cornea for the morphology and location of guttae, using inverted light microscopy.
Results
Guttae were seen as small, greenish, clustered bumps with some variation in size and color in peripheral host rims. None of the images of the peripheral host rims showed clear visibility of endothelial cells.
Conclusions
'Known guttae' in post-mortem FED eyesshowed a distinctive morphology and distribution in peripheral host tissue left in-situ, as compared to the grafted tissue centrally.