Relationship Between Il-10 Rs1800896, Tlr 9 Rs5743836 And Tnfa Rs1800629 Genes Polymorphisms And Different Genesis In Demodecosis Etiological Blepharoconjuctivitis.

There are several causes of vision system disorders and blindness, among which inflammatory diseases of the eye occupy the main place. The mechanism of the origin of inflammatory diseases in the eye area has not been thoroughly studied, and in this case, it occurs as a result of the complex effect of factors such as bacterial toxins, mechanical damage and immune dysfunction. Therefore, it is of great practical importance to identify polymorphisms that affect the expression and normal activity of TLR9, the proinflammatory cytokine TNFα and the anti-inflammatory IL10, which are important in the development of the innate immune response in the study of the mechanism of the development of inflammatory diseases of the visual system. 

Blepharoconjunctivitis - blepharitis and conjunctivitis are mutually complex manifestations and belong to the group of ophthalmological diseases. It is characterized by inflammation of the edge of the eyelids (blepharitis) and the surrounding conjunctiva (conjunctivitis). It is closely related to blepharitis and can be considered an advanced or aggravated form of blepharitis. 

78 patients with blepharitis or blepharoconjunctivitis of various genesis were examined for the purpose of scientific research and they formed the main group. Also, for the purpose of comparison, 50 conditionally healthy donors without blepharitis or blepharoconjunctivitis were examined. Blepharitis or blepharoconjunctivitis was detected in the examined patients using the 10th revision of the manual "International Classification of Diseases". According to it, chronic blepharoconjunctivitis occurred in 12 (15.4%) patients, blepharitis in 13 (16.7%) patients, acute inflammatory blepharoconjunctivitis and conjunctivitis in a total of 23 (29.5%) patients, demodectic blepharoconjunctivitis in 23 (29.5%) patients.

According to the examined polymorphisms, the IL-10 gene rs1800896 polymorphism in the main and control group was homozygous wild, heterozygous and homozygous mutant genotypes, respectively, 44 (71.8%) and 30 (60.0%); 18 (24.0%) and 17 (34.0%); 3 (3.8%) and 3 (6.0%) were detected in subjects, and in TLR 9 gene rs5743836 polymorphism, homozygous wild and heterozygous genotypes were 57 (77.0%) and 44 (88.0%); 8 (23.0%) and 6 (12.0%) patients with a homozygous mutant genotype were identified in the main and control groups, and 55 (85.9%) and 47 (94.0) patients with a homozygous wild-type and heterozygous genotype for the TNF-A gene rs1800629 polymorphism, respectively. ; 10 (14.0%) and 3 (6.0%) were obtained 

According to the results of the study,reliable association was found between patients with blepharoconjunctivitis disease of different genesis  and IL-10 gene rs1800896, TLR9 gene rs5743836 and TNFα gene rs1800629 polymorphisms.On the other hand,when patients were grouped according to the genesis of the disease,a reliable association between TLR9 gene rs5743836 polymorphism and blepharitis was found,compared to patients with the heterozygous genotype,the disease development was times.Increase was found.Thus,a statistically reliable correlation was found between TNFα gene rs1800629 polymorphism and the development of demodectic blepharoconjunctivitis and the disease development increased 4.35 times in patients with the heterozygous genotype.