Tear Liquid For Diagnosis Of Alzheimer Disease.
Published 2024 - 42nd Congress of the ESCRS
Reference: PO942 | Type: Free paper | DOI: 10.82333/fh7j-yr18
Authors: Salvatore Del Prete* 1 , Antonio Del Prete 2 , Mario Troisi 2 , Daniela, Ada Marasco 3 , Salvatore Troisi 4
1opthalmology,Service Biotech srl,napoli,Italy;Opthalmology,Service Biotech,Napoli,Italy, 2Opthalmology,University Federico II,Napoli,Italy, 3Opthalmology,Service Biotech,Napoli,Italy, 4Opthalmology,University of Salerno,Napoli,Italy
Purpose
The aim of our study was the identification with Immunocytochemistry of Amyloid Beta in tears from patients with
or without familiarity for Alzheimer disease, in order to make the diagnosis earlier and more accessible compared to other
invasive methods.
Setting
The common approaches at the diagnosis of Alzheimer Disease(AD) is made with an analysis of the
cerebrospinal fluid or the last techniques use the study of retinal fundus and the plaques formation, through OCT or more
simply with a fundus camera. Tears’ analysis is widely discussed in literature as an essential method to describe
molecular and biochemical alterations in different diseases.
Methods
Our study was performed on tears from three phenotypically healthy subjects: two of them were Caucasian
with Alzheimer familiarity (48 and 55 years old) and the other one was Asian without Alzheimer familiarity ( 45 years old)
and affected by an adenoviral keratoconjunctivitis at the moment of the withdrawal. Tears samples were collected from
eye fornix and were examinated by immunocytochemistry (ICC) assay using anti-Amyloid Beta antibody. Two out of
three tears samples showed positive Amyloid Beta.
Results
We observed that patient 1 and 2, with familiarity for Alzheimer, have Retinal plaques and presence Amyloid Beta residues on ICC samples, patient 3 not showed retinal plaques and not has Amyloid Beta residues in ICC. We have compared the samples 1, 2 and 3 with a negative control and positive control (1mg/5ml of Amyloid Beta) and we observe that the expression of Amyloid Beta in sample 1 and 2 is comparable to positive control and the sample 3 is comparable with negative control. We observed that the appearance of retinal plaques were directly linked with the presence of residues of Amyloid Beta in tears. We also note that the residues of beta is linked to expression of the retinal plaques.
Conclusions
Considering that our patients were phenotypically healthy, the identification of Amyloid
Beta by ICC could be a candidable method to make the diagnosis of the disease earlier and more accessible and
available then other current and invasive methods and it could be candidate to screening method too.