Relationship Between Of 25-Hydroxyvitamin D Level And Optical Biometric Indicators Of The Visual Analyzer In Myopia

to determine the level of 25-hydroxyvitamin D in the oral fluid of children with acquired myopia and its relationship with the biometric indicators of the visual analyzer.

This study was conducted in ZaporizhzhiaState Medical and Pharmaceutical University, Department of Ophthalmology, Medical Center "VISUS", Zaporizhzhia, Ukraine.

refractokeratometry, optical biometry (IOL Master 700, Carl Zeiss) with determination of axial length, as well as enzyme immunoassay for the level of 25-hydroxyvitamin D in oral fluid were performed with a set of reagents "25-HYDROXYVITAMIN D [25(OH)D] ELISA KIT" (cat. no. CAN-VD -510) produced by "Diagnostics Biochem Canada" in 13 children (26 eyes) with progressive myopia (I group), in 21 children (42 eyes) with stable myopia (II group). Control group – 18 healthy children (36 eyes) without ophthalmological pathology. Groups were representative in age, (11 to 16 years old).

The Student's t-test, the Kruskel-Wallis test, and the ROC analysis for determining the critical value of 25(OH) D were used to assess differences between groups.

In group I, the level of 25-hydroxyvitamin was 16.42 ± 2.62 ng/ml, which is 1.3 times lower than in group II and 2.3 times lower than in the control group, the data of which, respectively: 20 .95 ± 2.66 ng/ml and 39.25 ± 8.59 ng/ml (p<0.05). Correlation analysis showed a reliable inverse relationship between the level of 25-hydroxyvitamin D and the axial length of the eye (r= –0.62, p<0.05), as well as with the progressive course of myopia (r= - 0.79, p<0.05). According to the data of the ROC analysis, the optimal cut-off threshold values for the indicator of 25-hydroxyvitamin D in the oral fluid of children with myopia, which may indicate its progressive course, were determined: ≤ 20.15 ng/ml (sensitivity 94.1 %, specificity 94.1 %).

The obtained data indicate that the progression of myopia in children is associated with decreasing of 25-hydroxyvitamin D level in 76 % of cases. Determination of the level of 25-hydroxyvitamin D in oral fluid ≤ 20.154 ng/ml allows diagnosing the progression of myopia and can be used as an additional biomarker of the course of myopic refractogenesis in the practical work of an ophthalmologist.