ESCRS - FP02.02 - Kerato Biomechanics Study (1): A Comparative Evaluation Using Brillouin Microscopy And Dynamic Scheimpflug Imaging.

Kerato Biomechanics Study (1): A Comparative Evaluation Using Brillouin Microscopy And Dynamic Scheimpflug Imaging.

Published 2024 - 42nd Congress of the ESCRS

Reference: FP02.02 | Type: Free paper | DOI: 10.82333/ceeq-vv95

Authors: Riccardo Vinciguerra* 1 , Paolo Vinciguerra 1

1Humanitas University,Rozzano,Italy

Purpose

This study aims to assess the corneal biomechanical properties in normal and keratoconus patients (KC) using Brillouin microscopy (BOSS, Intelon, USA) and compare them with an ultra-high-speed Scheimpflug imaging (Corvis ST, Oculus, Germany).

Setting

Humanitas Clinical and Research Center

Methods

Sixty eyes from 60 patients (30 normal and 30 KC) were included in this prospective, single-center, comparative, non-interventional study. Corneal biomechanics were evaluated using Corvis ST and BOSS. With the BOSS system, each cornea was acquired 3 times, measuring 10 locations within an 8-mm diameter. Parameters extracted included Mean, Max, and Min Brillouin Shift (mBFS). These 10 points were also grouped into superior, central, and inferior regions. BOSS repeatability was assessed using the coefficient of repeatability (CR) and coefficient of variation (CV). Furthermore, we compared normal and KC patients using Corvis and BOSS.

Results

BOSS exhibited very good repeatability, with CR ranging from 0.098 to 0.138 GHz for single points in healthy patients and 0.096 to 0.149 GHz for KC patients. Statistical analysis revealed significant differences between normal and KC patients, indicating softer corneas in KC, observed with both Corvis and BOSS. Specifically, BOSS showed significant differences in mean, inferior, and superior MBFS (all p < 0.05), while Corvis displayed highly significant differences in Stiffness Parameter A1, Stress Strain Index, Deformation amplitude ratio, and Integrated radius (all p < 0.001).

Conclusions

Corneal biomechanical measurements proved highly repeatable and effectively demonstrated significant differences between normal and KC patients using both BOSS and the Corvis ST system.