DETECTION OF EPSTEIN-BARR VIRUS AND OTHER BACTERIAL AGENTS IN OCULAR ADNEXA MUCOSA-ASSOCIATED LYMPHOID TISSUE LYMPHOMA IN CHINESE
Published 2024 - 28th ESCRS Winter Meeting
Reference: FP37 | Type: Submission | DOI: 10.82333/2xgn-7h13
Authors: Chun Hin Yung* 1 , Jingna He 2 , Yan Tong 2 , Wai Kit Chu 2 , Allie Lee 3
1Li Ka Shing (LKS) Faculty of Medicine,The University of Hong Kong,Hong Kong,Hong Kong, 2Department of Ophthalmology and Visual Sciences, Faculty of Medicine,The Chinese University of Hong Kong,Hong Kong,Hong Kong, 3Department of Ophthalmology, School of Clinical Medicine, LKS Faculty of Medicine,The University of Hong Kong,Hong Kong,Hong Kong
Purpose
Ocular adnexa mucosa-associated lymphoid tissue (MALT) lymphoma is one of the most common cancers affecting the ocular surface. The role of bacterial and viral agents in the etiology of ocular adnexa MALT lymphoma has been the subject of on-going research, as results thus far remained inconclusive with significant geographical variability. This study set out to investigate the presence of Epstein-Barr virus (EBV), Chlamydia species, and Helicobacter pylori in ocular adnexal MALT lymphoma in a Chinese population.
Setting
This was a polymerase chain reaction (PCR) based analysis of biopsy samples obtained from ophthalmology centers in four hospitals (Queen Elizabeth Hospital, Hong Kong Eye Hospital, Queen Mary Hospital and Grantham Hospital) serving different regions of Hong Kong, where majority of the population are ethnic Chinese.
Methods
Cases of ocular adnexa MALT lymphoma with biopsy performed in the four hospitals between 2000 and 2019 were identified. Cases with incomplete or non-retrievable records or pathological samples were excluded. Eligible cases were histologically proven and validated by experienced pathologists. Archival formalin-fixed and paraffin-embedded (FFPE) samples were retrieved. Triplicate real-time quantitative PCR were performed using primers for EBV, Chlamydia psittaci, Chlamydia trachomatis, Chlamydia pneumoniae, and Helicobacter pylori.
Results
A total of 17 archival samples of ocular adnexa MALT lymphoma entered laboratory analysis. Chlamydia pneumoniae DNA was detected in one sample (5.9%). EBV, Chlamydia psittaci, Chlamydia trachomatis and Helicobacter pylori were negative in all samples.
Conclusion
In our study, no viral or bacterial agents were consistently detected in our Chinese ocular adnexal MALT lymphoma samples. The use of antimicrobial agents, which has emerged as an alternative treatment in some areas, should thus be considered with extra caution in this patient population.