Evaluation Of Antimicrobial Efficacy Of The «Novacel Ziyo» Ocular Medical Film In The Treatment Of Inflammatory Diseases Of The Anterior Surface Of The Eye
Published 2023 - 41st Congress of the ESCRS
Reference: PP02.15 | Type: Free paper | DOI: 10.82333/71h2-jr80
Authors: Dilbarhon Makhkamova 1 , Zebiniso Niyazova* 1 , Lyubov Khegay 2 , Mehridin Aslonov 3 , Zulfiya Shamsutdinova 1
1ophthalmology,Republican Specialized scientific practical center of eye microsurgery,Tashkent,Uzbekistan, 2ophthalmology,Republican Specialized scientific practical center of eye microsurgery,Tashkent,Uzbekistan;ophthalmology,Tashkent Medical Academy,Tashkent,Uzbekistan, 3ophthalmology,Tashkent Medical Academy,Tashkent,Uzbekistan
Purpose
To study the antimicrobial efficacy of an ocular medicinal film for the treatment of inflammatory diseases of the cornea on meat-peptone agar (MPA) and to evaluate the relationship of colony growth with the duration of the bio-coating.
Setting
To adapt to the treatment of inflammatory diseases of the anterior segment of the eye, we used a multifunctional bioactive film with antimicrobial property "Novacel Ziyo". The film coating is characterized by the creation of an optimal microenvironment for wound healing, antibacterial activity, high absorption capacity, permeability to gases, water vapor, photodynamic therapy, elasticity, absence of irritating, allergenic, pyrogenic and toxic effects.
Methods
The studies were carried out on 12 rabbits weighing 2.8-3.5 kg. The animals were divided into 2 groups: group I – the main group (6 individuals) - after modeling the infection of the eyes, the wounds were sutured, and a film was applied over the seams. Group II – control group (6 individuals) - after modeling eye infection, wounds were sutured and no bio-coating was applied. Rabbits of both groups were injected with Staphylococcus aureus (MRSA) culture into the affected eyes. The observation was carried out for 1, 3, 5 days.
Results
The dynamics of a decrease in the number of Staphylococcus aureus colonies by 4.5 times and their diameter by 1.08 times, as well as an increase in the lysis zone by 1.25 times in the main group indicates a long-term bacteriostatic effect of the developed biodegradable coating. The antimicrobial effect begins to manifest itself from 3 days, reaching its maximum on 5-7 days.
Conclusions
The results of in vitro studies confirm the specific bactericidal activity of the ophthalmic film for the treatment of penetrating eye wounds.