Corneal Keratoplasties Clinical Outcomes After Donor Cornea Hypothermic Storage In Eusol-C
Published 2023 - 41st Congress of the ESCRS
Reference: PO0657 | Type: Free paper | DOI: 10.82333/1z0m-kx21
Authors: Augusto Pocobelli 1 , Rossella Anna Maria Colabelli Gisoldi 1 , Eleonora Napoli 1 , Gemma Lodato 1 , Laura Giurgola 2 , Umberto Rodella 2 , Claudio Gatto 2 , Jana D'Amato Tothova* 2
1U.O.C. Oculistica - Banca degli Occhi,Azienda Ospedaliera S. Giovanni Addolorata - Roma,Roma,Italy, 2Research and Development,Al.CHI.MI.A. S.r.l.,Ponte San Nicolò,Italy
Purpose
To evaluate the clinical outcomes in patients after keratoplasties using donor corneas stored in Eusol-C storage medium at 2-8°C up to 14 days.
Setting
A retrospective study investigated 92 keratoplasties including Perforating Keratoplasty (PK), Descemet Membrane Endothelial Keratoplasty (DMEK), Descemet's Stripping Endothelial Keratoplasty (DSAEK), Deep Anterior Lamellar Keratoplasty (DALK) performed at the San Giovanni Addolorata of Rome using human donor corneas stored in Eusol-C (AL.CHI.MI.A. S.r.l.) at the Eye Bank of Rome at 2-8°C up to 14 days.
Methods
Donor sex and mean age, endothelial cell count (ECD), endothelial cell mortality (ECM), endothelial cell morphology score and corneal storage time were evaluated during storage in Eusol-C. The follow-up parameters after surgery included the presence of complications and corneal transparency 24 hours post-surgery (n=92) and the evaluation of visual outcomes and corneal transparency at 3 (n=15) and 6 (n=28) months post-surgery.
Results
Most donors had a mean age of 65±11 and were mainly male (57.6%). Corneas had a mean Eusol-C storage time of 7.7±2.5 days, with an ECD of 2398±354 cells/mm2 and a mean endothelial morphology score of 3.4±0.7. 28% of corneas lacked ECM while the other tissues showed on folds and iatrogenic ECM mainly. DSAEK was the most common surgical procedure (38%), followed by PK (32%), DALK (20%), and DMEK (11%). At 24 h post-surgery, 81% of patients had transparent, 17% partially transparent, and 2% oedematous corneas with no complications observed. Visual acuity was 6.4±3.6/10 and 6.7±2.8/10 at 3- and 6-months post-surgery, respectively. No cases of graft failure were observed up to 3 months and one graft rejection occurred at 6 months of follow-up.
Conclusions
Satisfactory surgical outcomes could be achieved utilizing donor corneas that have been stored in Eusol-C media for up to 14 days, as no adverse events or complications were observed within 6 months of follow-up.