Enhanced Transepithelial Corneal Cross-Linking Using A Novel Nano-Biomaterial
Published 2023 - 41st Congress of the ESCRS
Reference: FP02.11 | Type: Free paper | DOI: 10.82333/drsw-ck05
Authors: Jinhai Huang* 1 , Mei Yang 1 , Colm McAlinden 2 , Keith M. Meek 3 , Xingtao Zhou 1
1Eye Institute and Department of Ophthalmology, Institute for Medical and Engineering Innovation, Eye & ENT Hospital, Fudan University; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences,Shanghai,China;Shanghai Research Center of Ophthalmology and Optometry,Shanghai,China, 2Department of Ophthalmology, Singleton Hospital, Swansea Bay University Health Board,Swansea,United Kingdom, 3Structural Biophysics Research Group, School of Optometry and Vision Sciences, Cardiff University,Cardiff,United Kingdom
Purpose
Hibiscus-like RF@ZIF-8NF composites was constructed with a purpose to achieve a good TE-CXL effect with the preservation of epithelium, providing a new strategy for the treatment of keratoconus.
Setting
This study was conducted at the Eye and ENT Hospital of Fudan University.
Methods
Using 5-phosphoric acid-riboflavin (RF), 2-methylmimi and zinc nitrate as raw materials, RF@ZIF-8 composites was obtained by adjusting reaction conditions. The material charaterization, in vitro and in vivo biological evaluations of the obtianed RF@ZIF-8 composites were carried out in detail.
Results
The doping of RF in appropriate range has little effect on the phase structure of ZIF-8, while the morphology and size of the composites can be adjusted by changing reaction factors. Under same condication, kinds of experimental results indicated that the obtained RF@ZIF-8 composites has excellent corneal permeability, the RF content in the stroma was higher than that in other groups. The biocompatibility evaluation showed that the RF@ZIF-8 NF nanocomposites had excellent biocompatibility and no obvious damage to cornea. The in vivo TE-CXL effect of the RF@ZIF-8 composite was comparable to, or even slightly better than that of SCXL.
Conclusions
The constructed hibiscus-like RF@ZIF-8NF composites have excellent TE-CXL effect with epithelium preservated, its TE-CXL effect is comparable to that of SCXL, or even slightly better than that. It is expected to become a new type of TE-CXL nano-drug, providing new strategy for the treatment of keratoconus, which has high scientific research value, opens up a new direction for the development of CXL.