AUTO GRAFT LIMBAL STEM CELL, AMNIOTIC MEMBRANE AND AUTO SERUM EYE DROPS FOR SEVER CHEMICAL BURN WITHOUT KERATOPLASTY

To show that the effect of using  auto graft limbal stem cell and amniotic membrane and auto serum eye drops to reach clear cornea post sever chemical burn without the  need to do keratoplasty 

The MIOR

Prospective interventional study of (10) eyes of 10 patients (7males and 3 female) aged between 20-53 years with unilateral chemical burn .Patients were done by 1 surgeon in the memorial institute for ophthamic research (MIOR) with follow up period ranges from 6 m -1years.Inclusion Criteria : patients with unilateral chemical burn .Excluison Criteria : patients with bilateral chemical burn. Surgical Technique :  , I excised the adhesions and  fibrous tissue on the surface of the cornea , prepare the stem cells from other eye from superior part of normal cornea and divided it into 2 equal pieces then move them to the other eye and suturing them at the sides of the cornea at zero and 180 degree then fashion a large layer of the fresh amniotic membrane that cover the cornea and large area of sclera and suture the amniotic to the sclera , then suture the residual parts of conjunctiva above the amniotic to keep the amniotic membrane in place for long time .PreOperative :full ophthalmic examination specially visual acuity , anterior seg OCT , U/S .post operative:  signs of improvement as decrease corneal vascularization, clarity of cornea ,UCVA, BCVA, pentacam, Autoserum eye drops .

60% of eyes treated with auto graft limbal stem cell , amniotic membrane and auto serum eye drops  reach clear cornea post sever chemical burn without the  need to do keratoplasty and this take from 9 months to 1 year . 

 Chemical burn to the eye usually have a very poor prognosis

Limbal stem cell transplantation is the only hope for those patient who have massive destruction of their original stem cells

Use the amniotic membranes  and auto serum eyedrops  help the healing of these cases and some times give unexpected marvelous results that the corneas become almost clear and  we donot need to do keratoplasty but this need long time