ESCRS - PP05.16 - Examination Of Corneal Stromal And Regenerative Cells By Immunofluorescence Method After Keratoconus Treatment.

Examination Of Corneal Stromal And Regenerative Cells By Immunofluorescence Method After Keratoconus Treatment.

Published 2022 - 40th Congress of the ESCRS

Reference: PP05.16 | Type: Case report | DOI: 10.82333/rt26-8082

Authors: Njomza Hima Musa* 1 , Faruk Semiz 1 , Gamze Tanriverdi 2 , Zekeriya Alp Demirsoy 3 , Ceren Ece Semiz 4 , Olcay Semiz 1 , Anita Syla Lokaj 1

1Ophthalmology,Eye Hospital,Pristina,Albania, 2Department of Histology and Embryology,Istanbul University - Cerrahpasa Medical Faculty,Istanbul,Türkiye, 3Yeditepe,University of Medicine,Istanbul,Türkiye, 4Gazi ,University of Medicine,Ankara,Türkiye

To examine both the patient's cornea and the implanted lenticule with the immunofluorescence method three years after the lenticule was implanted with a SMILE in a patient with Keratoconus

Eye Hospital

Pristina

Republic of Kosovo

 

Methods:

A 20-year-old male patient with Keratoconus, who indicated a keratoplasty three years ago, had a right corneal central thickness of 402 μm and a vision of UDVA,0.1, CDVA,0.2,k1:46,k2:52.We implanted a 120 μm thick intrastromal fresh human lenticule with the SMILE method three years ago, and we followed up for this patient for one month, six months, one year, two years, three years. At the end of 3 years, we removed the implanted lenticule total; at the same time, we took a 20 μm piece of the patient's cornea. At the same time, we implanted 140 μm fresh lenticule intrastromal.

Results:

20 μm piece of the patient's cornea and removed the implanted tota lenticule; the pieces were examined by the immunofluorescence method. Well-organized parallel layered structures were seen in samples from both fragments, and healthy keratocytes and regenerative cells and stem cells,telocytes were observed. Thus, telocytes can be activated by appropriate stimuli such as stem cells and be involved in stromal regeneration

 

Fresh myopic intrastromal lenticular implantation is a safe, reliable technique that leads to increased corneal thickness, improved visual acuity, and the regeneration of healthy keratocytes ,telocytes, and it contains stem cells that are involved in stromal regeneration.