ESCRS - PP02.12 - Assessment Of The Concentrations Of Matrix Metalloproteinases-2 And Matrix Metalloproteinase-3 In The Corneal Epithelium In Patients With Cogan’S Microcystic Dystrophy.

Assessment Of The Concentrations Of Matrix Metalloproteinases-2 And Matrix Metalloproteinase-3 In The Corneal Epithelium In Patients With Cogan’S Microcystic Dystrophy.

Published 2022 - 40th Congress of the ESCRS

Reference: PP02.12 | Type: ESCRS 2022 - Posters | DOI: 10.82333/ycrr-fm44

Authors: Katarzyna Jadczyk-Sorek* 1 , Beata Bubała-Stachowicz 2 , Ewa Mrukwa-Kominek 3

1Ophthalmology,Professor K. Gibiński University Clinical Center, Medical Univeristy of Silesia ,Katowice,Poland, 2Ophthalmology,Professor K. Gibiński University Clinical Center Medical Univeristy of Silesia,Katowice,Poland, 3Ophthalmology,Professor K. Gibiński University Clinical Center, Medical Univeristy of Silesia,Katowice,Poland;Ophthalmology,Faculty of Medical Sciences, Medical University of Silesia,Katowice,Poland

Purpose

Assessment of the differences in the concentration of  matrix metalloproteinase-2 and matrix metalloproteinase-3 in the corneal epithelium between patients Cogan's microcystic dystrophy and patients with stable epithelial-stromal interface.

Setting

Matrix metalloproteinases are a group of proteolytic enzymes. They have the ability to degrade the elements of the extracellular matrix which contribute to faster cell migration and the release of molecules whose extracellular matrix is a reservoir. It is suspected that increased concentration of matrix metalloproteinases can cause instability of corneal epithelium and have influence on recurrent corneal erosions in patients with Cogan's basement membrane dystrophy.

Methods

The study group included patients with recurrent corneal erosions due to Cogan's basement membrane dystrophy qualified for the phototherapeutic keratectomy procedure (n=22 eyes). The control group included people with a stable epithelial-stromal interface who underwent Epi-Bowman Keratectomy (n=65 eyes).  Matrix metalloproteinases concentration was determined by immunohistochemical method using Human Magnetic Luminex® Assay.

Results

Assessment of the concentration of matrix metalloproteinase-2 and matrix metalloproteinase-3 in the corneal epithelium revealed: (1) statistically significant higher concentration of matrix metalloproteinase-2 in the group of patients with Cogan’s microcystic dystrophy compared to the control group;  (2) statistically significantly higher concentration of matrix metalloproteinase-3 in the group of patients with Cogan’s microcystic dystrophy compared to the control group.

Conclusions

The results can prove that matrix metalloproteinase-2 and matrix metalloproteinase-3 having the ability to dissolve anchoring fibers and the corneal epithelial basement membrane could be responsible for recurrent corneal erosions in a course of  Cogan’s microcystic dystrophy.