ESCRS - P158 - PERSPECTIVES FOR THE DEVELOPMENT OF PRECLINICAL MOUSE MODELS OF GLAUCOMA AFTER BOSTON KERATOPROSTHESIS TYPE 1

PERSPECTIVES FOR THE DEVELOPMENT OF PRECLINICAL MOUSE MODELS OF GLAUCOMA AFTER BOSTON KERATOPROSTHESIS TYPE 1

Published 2022 - 26th ESCRS Winter Meeting

Reference: P158 | Type: Abstracts | DOI: 10.82333/c6w0-b712

Authors: Dominique Geoffrion* 1 , Marie-Claude Robert 2 , James Chodosh 3 , Adriana Di Polo 4 , Mona Harissi-Dagher 2

1Faculty of Medicine,McGill University,Montreal,Canada, 2Department of Ophthalmology,Centre Hospitalier de l'Université de Montréal (CHUM),Montreal,Canada, 3Department of Ophthalmology,Massachusetts Eye and Ear, Harvard Medical School,Boston,United States, 4Department of Neurosciences,Centre de recherche du Centre hospitalier de l'Université de Montréal (CHUM),Montreal,Canada

Purpose

To determine the feasibility of establishing a reproducible mouse model of glaucoma after Boston keratoprosthesis type 1 (KPro) surgery, specifically that of a miniaturized mouse model of KPro (mKPro).

Setting

Centre de recherche du Centre hospitalier de l'Université de Montréal (CR-CHUM), Montreal, QC, Canada

Methods

A total of 20 corneas of donor C57BL/6 mice (n=10) were implanted in one eye of each recipient BALB/C mice (n=20), assembled as part of the mKPro, either with or without intraoperative lensectomy. Main feasibility outcomes consisted in incidence rates of hypotony, capsule nicking, and lens extrusion, as well as acquisition of posterior segment OCT images.

Results

With lensectomy (n=10), loss of ocular tone and rhegmatogenous retinal detachment occurred in 100% of mice. Without lensectomy (n=10), capsule nicking and opening, as well as lens extrusion, occurred in 80% of mice. Causes of these complications included the large proportion of intraocular volume occupied by the lens, the shallow anterior chamber, and thus the lack of available intraocular volume to implant the KPro if the lens remains present.

Conclusion

Successful mKPro surgery may require a great deal of practice to be useful as a reproducible model in mice. An animal model with a larger eye ought to be prioritized by research teams in future studies.