A Pilot Study Of Lyophilized Decellularized Storage Of Smile-Derived Lenticules
Published 2025 - 43rd Congress of the ESCRS
Reference: FP14.15 | Type: Free paper | DOI: 10.82333/wg0n-0g20
Authors: Nino Hirnschall** 1 , Klemens Waser 1 , Klaus Strassmair 1 , Sophia Reifeltshammer 1 , Leon Pomberger 1 , Matthias Bolz 1
1Kepler University Clinic And Johannes Kepler University,Linz,Austria
Purpose
This study aimed to assess the feasibility and biocompatibility of a novel approach for preserving small incision lenticule extraction (SMILE) surgery-derived lenticules by integrating decellularization and lyophilization techniques.
Setting
The in-vitro measurement on lenticule transparency, structural integrity, and biocompatibility of the lyophilized decellularized storage of SMILE-derived lenticules.
Methods
A total of 72 lenticules were divided into two distinct groups: the lyophilized decellularized storage group, which underwent treatment with Triton X-100 and SDS, followed by freeze-drying and storage at -20°C for 24h; and the control group, which was stored in a sterile plastic vial at room temperature post-extraction. Comprehensive analyses, including scanning electron and transmission electron microscopy, hematoxylin-eosin staining, transmittance measurements, and live/dead staining, were conducted on all lenticules to evaluate their preserved properties. SPSS v20.0 was used for statistical analysis of quantitative data, and P<0.05 was considered statistically significant.
Results
Histomorphology observed that the lyophilized decellularized lenticules effectively removed cellular components while preserving the structural regularity and integrity of the collagen fibers. After rehydration, the lenticules exhibited central thickening and peripheral thinning. The optical transmittance of all rehydrated lyophilized decellularized lenticules was significantly higher than that of lyophilized ones (all P < 0.05), but still lower than that of fresh lenticules (all P < 0.05). After 20-30 minutes of rehydration, the optical transmittance of the lenticules exceeded 80%, and both weight and optical transmittance stabilized (all P > 0.05). Furthermore, in vitro biocompatibility testing revealed no detection of dead cells.
Conclusions
Lyophilized decellularized storage of SMILE-derived lenticules effectively preserved the tissue morphology, optical transparency, and biocompatibility, suggesting potential for clinical application.