Corneal Biomechanical Outcome Of In Vivo Enzymatic Collagen Crosslinking In Rabbits Evaluated By Scheimpflug Technology
Published 2023 - 41st Congress of the ESCRS
Reference: PP14.05 | Type: Free paper | DOI: 10.82333/fv78-9c18
Authors: Yuniar Sarah Ningtiyas* 1 , Puspita Sitorasmi 1 , Yulia Primitasari 1 , Dicky Hermawan 1 , Luki Indriaswati 1
1Department of Ophthalmology,Dr. Soetomo General Academic Hospital,Surabaya,Indonesia;Faculty of Medicine,Universitas Airlangga,Surabaya,Indonesia
Purpose
This study aimed to investigate the biomechanical outcome of in vivo enzymatic (transglutaminase) corneal crosslinking in rabbit cornea employing Scheimpflug technology (Corvis ST), providing a potential approach for the treatment of corneal ectasia.
Setting
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Methods
A total of 21 white New Zealand rabbits were separated into three groups: transglutaminase crosslinking (CXL), epithelial-off CXL, and transepithelial CXL. The corneal surface was treated with a 1 U/mL solution of microbial transglutaminase, and both the epithelial-off and transepithelial groups were treated with clinical ultraviolet A-riboflavin (UVA/RF). Using Scheimpflug imaging, corneal biomechanical parameters were assessed 14 days following the procedure. New Corvis ST (Oculus Optikgeräte GmbH, Wetzlar, Germany) parameters such as stiffness parameters at first applanation (SPA-1), deformation amplitude (DA) ratio, integrated radius (IR), and stress-strain index (SSI) were also assessed.
Results
The biomechanical outcome of transglutaminase CXL was comparable to photochemical CXL. Among the Corvis ST parameters, the radius at highest concavity exhibited significant differences among the three groups. The highest radius was observed in the epithelial-off group (5.21 ± 0.81 mm) whereas the transglutaminase CXL group had the lowest radius (4.04 ± 0.52 mm). DA was lowest in the transglutaminase CXL group (0.20 ± 0.14 mm). As for the latest Corvis ST parameters, SPA-1 and SSI were found highest in the transglutaminase CXL group with values of 33 (17.4, 54.4) mmHg/mm, 0.97 ± 0.37, respectively. These new parameters showed no significant difference among groups.
Conclusions
Transglutaminase CXL and UVA/riboflavin CXL outcomes in rabbit cornea are biomechanically equivalent, as indicated by our findings. However, additional investigations are required to evaluate the significance and safety of this potential therapeutic approach in halting the corneal ectasia progression.