ESCRS - PP07.12 - In Vitro Evaluation Of The Antiseptic Power Of Eight Ophthalmic Preparations As A Strategy To Hamper The Antibiotic Resistance

In Vitro Evaluation Of The Antiseptic Power Of Eight Ophthalmic Preparations As A Strategy To Hamper The Antibiotic Resistance

Published 2023 - 41st Congress of the ESCRS

Reference: PP07.12 | Type: Free paper | DOI: 10.82333/e42f-sa14

Authors: Carlo Cagini 1 , Michael Spinosi* 1 , Francesco Della Lena 1 , Maria Pia Carafa 1 , Daniele Marini 2 , Carla Russo 2 , Donatella Pietrella 2

1Department of Medicine and Surgery, Ophthalmology Section,S. Maria della Misericordia Hospital, University of Perugia, Italy,Perugia,Italy, 2Department of Medicine and Surgery, Microbiology Unit,University of Perugia, Italy,Perugia,Italy

Purpose

To evaluate the in vitro antiseptic power of eight ophthalmic preparations to prevent the occurrence of eye infections without the use of antibiotics.

Setting

Medical Microbiology Unit,  University of Perugia,  Italy

Methods

A total of 8 ophthalmic solutions (Corneial Med, Désomédine, Iodim, Keratosept, Ocudox, Oftasecur, Ozodrop, Visuprime) was tested against two strains of gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis), one strain of gram-negative bacteria (Pseudomonas aeruginosa) and one strain of yeast (Candida albicans). The effect of the eye drops on bacterial cultures and yeast cells was evaluated by the Minimum Inhibitory Concentration assay and growth kinetics analysis.

Results

The ophthalmic solutions were effective in reducing the bacterial or cellular load of all microorganisms tested in vitro with similar values to the positive control. In particular, Visuprime has shown the best antimicrobial activity, proving to be able to inhibit or slow down the growth of microorganisms even at low concentrations.

Conclusions

The antiseptic power of ophthalmic preparations tested in vitro is effective against classes of microorganisms responsible for eye infections. The prolonged antimicrobial activity observed in vitro at high dilutions suggests a possible use of antiseptics in vivo, which should retain their activity even after dilution with tears