ESCRS - PO0920 - Proteomic Changes In Aqueous Humour, Anterior Capsule And Crystalline Lens In Diabetic And Age-Related Cataract.

Proteomic Changes In Aqueous Humour, Anterior Capsule And Crystalline Lens In Diabetic And Age-Related Cataract.

Published 2023 - 41st Congress of the ESCRS

Reference: PO0920 | Type: Free paper | DOI: 10.82333/f3z2-xn92

Authors: Christina Karakosta* 1 , Martina Samiotaki 2 , Anastasios Bisoukis 3 , Dimitrios Papakonstantinou 1 , Marilita Moschos 4

11st University Eye Clinic,National and Kapodistrian University of Athens, "G. Gennimatas" General Hospital of Athens,Athens,Greece, 2Biomedical Sciences Research Center "Alexander Fleming",Vari,Greece, 3Ophthalmology Department,Athens General Hospital 'Evangelismos',Athens,Greece, 4Electrophysiology of Vision,1st University Eye Clinic of Athens,Athens,Greece

Purpose

To present distinct signaling pathways involved in the development of age-related cataract (ARC) and diabetic cataract (DC).

Setting

1st University Eye Clinic, “G. Gennimatas” General Hospital of Athens, National and Kapodistrian University of Athens, in cooperation with Biomedical Sciences Research Center “Alexander Fleming”, Vari, Attika, Greece.

Methods

Three sample groups, e.g. the aqueous humour, the anterior capsule and the content of the phaco cassette, were collected during cataract surgery.  The samples were collected from 11 patients with diabetes mellitus, younger than 65 years old, and from 12 patients without diabetes mellitus, older than 75 years old. The samples were prepared based on Sp3 protocol (Single-Pot, Solid-Phase-enhanced, Sample-Preparation). The recognition and quantification of proteins was performed with liquid chromatography online with tandem mass spectrometry. Data analysis was performed using the DIA-NN software for identification and quantification of peptides and proteins. Perseus software was used for statistical analysis and data visualization.

Results

Wnt signaling pathway was significantly upregulated in the content of the phaco cassette samples of ARC, while pentose phosphate pathway was upregulated in those of DC. Glycosphingolipid and glycosaminoglycan biosynthesis pathways were significantly upregulated in all three samples of ARC. Pathways involved in keratinocyte differentiation, migration and proliferation were upregulated in anterior capsule and aqueous humour samples of DC.

Conclusions

Outstanding proteomic profiles of the three sample types have been generated using DIA proteomics. Grouping of the patients allows deciphering of numerous deregulated pathways correlated with the disease. The generated data sets may be useful in finding protein druggable targets against cataract.