The Interaction Of Sodium Hyaluronate (1.0% ) With Bacteria Strains And Antibiotics: An In Vitro Study

To evaluate the interaction of sodium hyaluronate 1.0% ophthalmic viscosurgical device with standard bacteria strains and antibiotics.

BURSA ULUDAG UNIVERSITY, FACULTY OF MEDICINE OPHTHALMOLOGY AND MICROBIOLOGY DEPARTMENT

Five in vivo experimental study steps  were created with four standard bacteria strains. Cefuroxime or moxifloxacin were used as an antibiotics. In first step sodium hyaluronate 1.0% and bacteria strains; in  second step sodium hyaluronate 1.0%, bacteria strains and antibiotics were studied. In the third experimental step, in addition to the second  step, irrigation was done. In the fourth and fifth experimental steps, both bacterial and antibiotic retention of sodium hyaluronate 1.0% was evaluated separately. Cultivation was done on the  sheep blood agar medium. Each experimental steps were repeated three times and averaged. Logarithmic changes in colony numbers between the first step and other steps were calculated.

Staph aureus and Staph epidermidis (with and without biofilm) and Pseudomonas aeruginosa strains were examined. In second step colony numbers were significantly reduced and there was more decrement  with moxifloxacin when compared to cefuroxime. Maximum logarithmic decrease was seen in third step. In  fourth step, in which the interaction of sodium hyaluronate 1.0% with antibiotics was evaluated, colony numbers were significantly reduced for all bacteria, but this decrement was lower than in third step. In fifth step, in which the interaction of sodium hyaluronate 1.0%  with both bacteria and antibiotics was evaluated, a more decrement in colony numbers was observed in surface cultivation compared to bottom cultivation after centrifugation.

In this study, it was observed that sodium hyaluronate 1.0%  interacts with both bacteria and antibiotics. Administration of antibiotics in the presence of sodium hyaluronate may reduce antibacterial activity due to the antibiotic retention capacity. However, sodium hyaluronate can also adhere to bacteria, and it may cause bacterial remain for a longer time.