Information Registration Programme Overview Satellite Education Programme Virtual Exhibitor Listing


All ePoster presentations will be available to view anytime throughout the Meeting in an Online Library

Search Abstracts by author or title


Impact of temperature on the biomechanical effect in epithelium-off corneal cross-linking

Poster Details

First Author: H. Abdshahzadeh SWITZERLAND

Co Author(s):    R. Abrishamchi   E. Torres-Netto   N. Hafezi   J. Randleman   F. Hafezi        

Abstract Details


Oxygen diffusion plays a central role in the efficiency of corneal cross-linking (CXL). Oxygen availability within a tissue increases with reduced temperatures. The purpose of this study was to evaluate whether a reduction of the corneal temperature during CXL might increase oxygen availability and thus enhance the biomechanical effect of CXL in ex vivo porcine corneas.


Laboratory for Ocular Cell Biology, Center for Applied Biotechnology and Molecular Medicine, University of Zurich, Zurich, Switzerland and ELZA Institute, Dietikon/Zurich, Switzerland


One hundred twelve porcine corneas with intact epithelium were divided into 4 groups and analyzed. Prior to corneal soaking with hypo-osmolaric 0.1% riboflavin, the epithelium was removed manually in all groups. Accelerated epithelium-off CXL using 9 mW/cm2 irradiance for 10 minutes was performed either at room temperature (group 1, 24ᄚC) or in a cold room (group3, 4ᄚC). Non-cross-linked corneas (groups 2 and 4) were subjected to the same temperatures and served as controls. The elastic modulus of 5-mm wide corneal strips was analyzed and used to determine corneal biomechanical properties.


Epithelium-off CXL led to significant increases in the elastic modulus determined between 1% and 5% of strain in stress-strain extensometry when compared to non-cross-linked controls, both at 24ᄚC (p<0.001) and 4ᄚC (p=0.006) . However, no significant difference was found between corneas treated with CXL at 24ᄚC and 4ᄚC (p = 0.384).


While oxygen plays a central role in corneal cross-linking, the potentially increased diffusion of oxygen in lower tissue temperatures does not appear to play a significant role in the biomechanical efficiency of epithelium-off CXL accelerated protocols in ex vivo porcine corneas.

Financial Disclosure:


Back to Poster listing