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Laser spectroscopic study on getting best results with trypan blue dye staining of anterior lens capsule

Poster Details

First Author: M.Mathen INDIA

Co Author(s):                        

Abstract Details


To elucidate the molecular changes in anterior lens capsule after staining with different concentrations of trypan blue dye at various time intervals.


Chaitanya Eye Hospital and Research Institute


Anterior lens capsules collected after capsulorhexis from 20 patients. Capsules exposed to various time intervals and dilutions of dye. Raman laser spectrometry used to get spectra from unstained and stained capsules. Computerised data plotting done.


Dye stained capsules showed broadening of the secondary structure marker amide I band and appearance of higher wavenumber at 1690 cm-1 showing that capsule proteins undergo uncoiling from tightly packed triple helix to randomly coiled state. The 1 in 5 dilution (0.012%) stained capsule showed these changes after 15 sec of exposure time.


Trypan blue staining of anterior lens capsule makes it less elastic and stiffer. If dye is used as 1 in 5 (0.012%) dilution of the 0.06% solution with exposure time of 10s or less, capsule shows no structural changes.

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