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Modified 30G needle under air trypan blue (TB) dye anterior capsule staining technique vs under viscoadaptive substance staining technique

Poster Details

First Author: S.Mahajan INDIA

Co Author(s):    T. Om Parkash   R. Om Parkash                 

Abstract Details


To compare modified 30 G needle under air Trypan Blue (TB) dye staining technique with under viscoadaptive substance staining technique


Dr Om Parkash Eye Institute, Amritsar, India


In this case control prospective study, 100 eyes undergoing phacoemulsification were randomized into two groups. Group I (50 eyes) underwent trypan blue staining with modified 30 G needle staining technique and group II (50 eyes) underwent under viscoadaptive staining using 0.08% TB dye (Rhex ID, Appasamy ocular devices Pvt Ltd, India).


In group I, anterior chamber remained stable during the hold time of 15 seconds with no egress of air. The staining intensity was excellent in 81.2 % of the eyes and good in 18.8% of the eyes whereas in group II, staining intensity was excellent in 79.8 % and good in 20.2 % (p > 0.05). No iatrogenic trauma occurred in any of the cases. All cases had a homogeneously stained anterior capsule. Tyrpan blue used was more in group II as compared to group I.


Trypan blue staining under air using modified 30 G needle technique and under viscoadpative substance gives comparable homogenous staining of the anterior capsule. However, more drops of the dye are required while performing under viscoelastic substance staining.

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