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Biomechanical properties of different corneal cross-linking (CXL) irradiances

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Session Details

Session Title: Presented Poster Session: Cornea IV

Venue: Poster Village: Pod 3

First Author: : S.Chen CHINA

Co Author(s): :    Y. Zheng              

Abstract Details


To evaluate whether different Corneal Cross-Linking (CXL) Irradiances with a constant delivered total energy could achieve the same increase in corneal material stiffness based on corneal inflation test.


The Affiliated Eye Hospital, Wenzhou Medical University.


126 healthy white Japanese rabbits were randomly divided into 7 groups. All the left eys served as treat group,and all right eyes as normal group.6 groups were exposed to riboflavin 0.22% and UV-A(370nm) irradiationiation of equal total energy (3mW/cm 2 for 30 minutes, 9mw/cm2 for 3 minutes, 45 mW/cm 2 for 2 minutes,90 mW/cm 2 for 1 minute). The control eyes were exposed to riboflavin without irradiation. 12 corneas of each group were prepared for inflation test, while other 6 specimens were processed for electron microscopy to measure the fibril diameter and interfibril spacing.


The stiffening effect of CXL decreased when using high irradiation/short irradiation time settings. At 0.010 MPa stress stage, it showed significant differences between 3 mW group and the NUVA group (p <0.05), 9 mW group and the NUVA group (p <0.05). Compared with the NUVA group, the tangent modulus in 3 mW group was significantly increased by 212.5 %, the 9 mW group increased by 196.8 %.The interfibril spacing were significant lower in 3 mW group and 9mW group (p <0.05),However, fibril diameter kept the similar (p >0.05).


The effect of biomechanical properties stiffening and interfibril space narrowing of rabbit cornea underwent corneal collagen cross-linking decreased with increasing irradiance using inflation after a week. Therefore the Bunsen-Roscoe law might not be readily used in corneal collagen cross-linking. Increased oxygen consumption associated with higher irradiances may be a limiting factor leading to reduced treatment efficiency.

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