A new presurgical prophylaxis on a high microbial contamination in vivo model with bactericidal liposomal ozone dispersion
First Author: A.Marchegiani ITALY
Co Author(s): M. Magagnini M. Cerquetella P. Troiano I. Franchini A. Franchini A. Spaterna
The purpose of this study was to assess the effectiveness of preoperative liposomal ozone dispersion in reducing bacterial colonization from the ocular surface in dogs as a high-contamination microbial model, in comparison with povidone-iodine (PI). In addition, the effect of one-day preoperative prophylactic topical therapy with liposomal ozone dispersion in comparison with topical fluoroquinolones was tested. To exclude any possible effect of the excipients, the antimicrobial effect of the solely vehicle of the liposomal dispersion was also assessed.
Veterinary Teaching Hospital, School of Biosciences and Veterinary Medicine, University of Camerino.
Prospective, randomized, double-blind povidone-iodine controlled study. Twenty-two owned dogs consisting with 44 eyes in total scheduled for diversified ophthalmic surgical procedure were enrolled and divided in four groups (ozone, povidone iodine, fluoroquinolone, placebo) receiving the respective preoperative protocol. A swab was taken before beginning and at the end of the protocol evaluating total microbial count, coagulase positive and negative staphylococci. Specimens were processed in accordance with routine microbiology methods using neutralizing agents to eliminate the activity of interfering antimicrobial agents such as povidone iodine and ozone.
At baseline, there were no statistically significant differences between the groups in growth rates of any bacteria. Moderate eye irritation after prophylactic treatment with povidone-iodine were recorded in 4 dogs (punctate keratitis, corneal oedema); no other side effects were reported. Ozone group showed an optimal tolerability, having no topical side effects. Statistical analysis revealed a significant decrease in CFU for total microbial count, coagulase positive and negative staphylococci both for liposomal ozone dispersion and povidone iodine, similar in the two groups. No statistical difference were found in median CFU for both one-day placebo and fluoroquinolone preoperative prophylactic topical therapy.
The results of the present study demonstrate that liposomal ozone-dispersion is as effective as povidone iodine in reducing preoperative bacterial load in ocular surface of dogs. Interestingly, ozone showed a higher tolerability in comparison with povidone iodine. In view of this, liposomal ozone-dispersion could constitute an adjuvant and complementary approach to the only povidone iodine 5% used in the operating theatre or as home therapy in the days preceding surgery.