Evaluation of biocompatibility of cross-linked polyisobutylene (xPIB) IOL and its effect on preventing secondary cataract formation in vitro
First Author: W.Li CHINA
Co Author(s): H. Lin X. Fang B. Pan
To evaluate the biocompatibility of xPIB IOL (Xi’an Pillar Bioscience) and its effect on preventing posterior capsule opacification (PCO) while comparing its performance with commercially available hydrophobic acrylic IOL (Alcon Lab) in vitro.
1. School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China. 2. Xi’an Pillar Bioscience Co. Ltd, Xi’an, Shaanxi, China
Using HLEC-B3 cell line, the effect of the IOL extracts from each group on the cell proliferation was assessed by the CCK-8 method to verify its biological safety. The differences in morphology, adhesion and proliferation of surface-adhered LECs between the xPIB IOL and the hydrophobic acrylic IOL were evaluated with light-microscope, crystal violet staining and CCK-8 method, respectively. The mRNA expression levels of autocrine regulation related genes of LECs were assessed by RT-qPCR. Cell culture model that simulates the human eye capsule environment and rabbit eye model were used to evaluate the potential of effect xPIB IOL on preventing PCO.
The IOL extracts from each group showed no significant effect on the LEC proliferation; the LEC proliferation on xPIB surface was significantly slower than on acrylic one. The CCK8 and crystal violet staining showed cell adhesion (OD570nm, 0.17±0.01 vs. 0.54±0.03) and proliferation (OD450nm, 0.62±0.02 vs. 0.88±0.02) of LECs on xPIB IOL were remarkably lower than on acrylic IOL. The mRNA expression levels of α-SMA, TGF-β1, EGF, and bFGF in xPIB group increased than in acrylic group. The migration LEC coverage on IOL optic surface showed no statistical difference between the two groups (39.62%±3.93% vs. 43.85%±3.17% (p>0.05).
These results showed that LECs demonstrated less affinity to the surface of the xPIB IOL than to that of hydrophobic acrylic IOL. In addition, the stretch ability and proliferation of the LECs on the surface of the xPIB IOL were also lower than that on the surface of hydrophobic acrylic IOL. The results indicated that xPIB IOL possessed the similar to but potentially better ability of preventing the development of posterior capsule opacification than acrylic IOL. Further in vivo and clinical investigations are recommended to confirm the in vitro findings.
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