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Optimal parameters to improve the interface quality of the flap bed in femtosecond laser-assisted LASIK

Poster Details

First Author: S.Serrao ITALY

Co Author(s):    G. Lombardo   D. Schiano Lomoriello   G. Ducoli   M. Rosati   P. Ducoli   M. Lombardo

Abstract Details


To analyze the interface quality of the anterior stroma after femtosecond (FS) laser flap creation using Atomic Force Microscopy (AFM).


Fondazione G.B. Bietti IRCCS, Rome, Italy


A 110 µm depth flap was created in twenty human corneal tissues using a FS laser platform (Intralase iFS, Abbott Medical Optics). Tissues were divided into four groups of various cutting parameters: pulse energy and spot separation were respectively 1) 0.75 µJ and 6 µm, 2) 0.65 µJ and 5 µm, 3) 0.55 µJ and 4 µm and 4) 0.45 µJ and 4 µm. Four more tissues were cut using a motorized microkeratome (Hansatome, Bausch & Lomb). AFM (Autoprobe CP, Veeco) analysis was performed on the stromal bed of each sample.


The corneal tissues treated with higher pulse energies and wider spot separations (groups 1 and 2) showed a rougher stromal bed interface (RMS rough: 0.23 ± 0.008 µm and 0.24 ± 0.009 respectively) than tissues of groups 3 and 4 (RMS rough: 0.18 ± 0.006 µm and 0.18 ± 0.008 µm respectively; ANOVA: P<0.001). The stromal surface quality of tissues treated with pulse energies of 0.55µJ or lower and 4 µm spot separation compared favourably with that cut by the microkeratome (RMS rough = 0.17 ± 0.006 µm; Tukey: P>0.05).


The FS stromal interface quality was improved setting pulse energy lower and spot separations narrower than those currently used in the clinical setting. FS laser could provide a flap interface smoothness comparable to that provided by the microkeratome. FINANCIAL DISCLOSURE?: No

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