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Morphological diagnostics of ocular surface squamous neoplasia

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Session Details

Session Title: Imaging

Session Date/Time: Tuesday 16/09/2014 | 08:00-10:30

Paper Time: 09:56

Venue: Capital Hall B

First Author: : T.Valkovich BELARUS

Co Author(s): :                  

Abstract Details


To determine diagnostic potentialities of impression cytology in ocular surface squamous neoplasia diagnostics.


Belarusian Medical Academy Postgraduate Education, Minsk, Belarus


Investigation was carried out among 8 patients with ocular surface squamous neoplasia. Corneal epithelium was involved in pathological process in 5 patients. Both corneal and conjunctival epithelium were pathologically changed in 3 patients. Impression cytology was performed on all patients both on the zones of obvious disturbance and the zone without clinical changes. The received material was stained in accordance to hematoxylin and eosin protocol and light microscopy was performed using magnification ×20, ×40, ×63, ×100. Morphometry was carried out with help of Leica Application Suite. Immunological investigation on human immunodeficiency virus infection, human papillomavirus infection, cytomegalovirus infection and herpes virus infection was performed on all patients.


Epithelial hypertrophy was revealed in accordance with the clinical location of tumor. Epithelial cells were characterized by heterochromia of nuclei, polynucleoli, condensation of chromatin, decrease nucleus cytoplasm ratio aside increase dimensions of nucleus. This disturbance are conformed to III degree of dysplasia. Moreover, a lot of the «owl eye» cells were determined. These cells contained virus. The indications of mixed infection were observed in 2 patients. They had both signs of cytomegalovirus infection and human papillomavirus infection: the «owl eye» cells and cells with apically displaced and flattened nucleus and polynuclear cells. The same disturbances were determined throughout the whole of the investigated surface, not only the clinical location of tumor. Immunological investigation confirmed cytomegalovirus infection in all cases and mixed infection (cytomegalovirus infection and human papillomavirus infection) in 2 patients.


Ocular surface squamous neoplasia is serious disease of multifactorial in character which demands comprehensive approach in diagnostics and treatment. Obtained results can indicate the need for etiotropic therapy before and after surgical treatment and to expansion of surgical intervention zone. To determine the dimension of surgical treatment, impression cytology must be done before the beginning of treatment. Impression cytology is a high-quality and minimally invasive method of morphological investigation and it allows to confirm etiology of ocular surface squamous neoplasia and to choose conventional treatment. The further follow-up period will show the efficacy of antiviral treatment in these cases.

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