Lisbon 2017 Delegate Registration Programme Exhibition Virtual Exhibition Satellites OneWorld Travel Discount
escrs app advert


Search Title by author or title

Comparison of punching techniques for preparation of endothelial grafts for Descemet's membrane endothelial keratoplasty

Poster Details

First Author: R.Tan CANADA

Co Author(s):    P. Morales   M. Taha   E. Santiago   N. Noordeh   Y. Yang   K. Baig     

Abstract Details


To compare the use of a widely available dermatologic punch for preparing endothelial grafts for Descemet Membrane Endothelial Keratoplasty (DMEK), with the current standard trephine punch.


University of Ottawa Eye Institute, Ottawa, Ontario, Canada


This is a prospective, randomized, masked, ex-vivo paired-eye investigation. In a wet-lab setting, eleven pairs of donor corneas were harvested using the submerged cornea using backwards away (SCUBA) technique. For each pair, one cornea was prepared using a 10mm Barron punch (BP) and the other with a 10mm dermatologic Acu-punch (AP). The number of graft tears was counted for each punching technique. Also, the circularity of the grafts was evaluated though Adobe�Â�® analysis and the ease of peeling graft tissue graded using a 5-item Likert scale. The Mann-Whitney U and chi-square tests were used with significance set at p=0.05.


Results of 11 paired corneas revealed that the number of graft tears were not statistically different when using the BP (4.64 3.38) vs AP (4.46 6.15; U = 47.00, Z = 0.90, p = 0.37). Also, the circularity of the graft after using the BP (0.72�.095) and the AP (0.73�.11) were also statistically not different (U = 49.0, Z = -0.76, p = 0.45). No differences were found in the ease of peeling between the BP group and AP group (X2 = 0.277, df = 2, p = 0.871).


The widely available dermatologic punch (Acu-Punch) is comparable to the standard Barron punch when used for harvesting grafts for DMEK. It may be a cheaper, widely available alternative to the standard punch and could potentially make DMEK more affordable and widespread in the future.

Financial Disclosure:


Back to Poster listing