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Comparison of the effects of topical cyclosporine a and dexamethasone on an experimental model of allergic conjunctivitis

Poster Details

First Author: H.Cakmak TURKEY

Co Author(s):    A. Akyuz Unsal   F. Kiral   A. Bildik   T. Kocaturk   M. Serter   K. Ergin

Abstract Details

Purpose:

to compare the effects of topical 0.05% cyclosporine A and 0.1% dexamethasone on mast cells and allergic inflammatory mediators on an experimental model of allergic conjunctivitis at Balb/c mice.

Setting:

Pathogen-free 8-12 weeks old 24 Balb/c male mice were included in the study by the animal facility of the Adnan Menderes University Experimental Animal Production and Research Center. Mice were randomly divided into 4 groups as following:1) Allergy group (positive control),2) Dexamethasone group,3) Cyclosporine A group,4) negative control group.

Methods:

Mice were immunized with intraperitoneal ovalbumin and also on their eyes. The group 2; were administered 0,1% dexamethasone 2 times a day, group 3; 0,05% cyclosporine A 2 times a day, group 4; balanced salt solution 2 times a day topically. After the 24th hour of the last OVA challenge, all of the globes and eyelids of the mice were excised. The sections were stained by Hematoxylin-Eosin and Toluidine Blue. Anti-mouse monoclonal anti-core which was prepared against mast cell triptase was applied. At the end of the study; IL1A,IL3,IL-6,IL-10 and TNF-α serum levels of the mice determined.

Results:

Histopathology revealed inflammation, edema and vascular proliferation in allergy group and high mast cell tiptase (mct) counting. Pathologic findings disappeared and mct counting was low at dexamethasone and cyclosporine A groups. Serum levels of IL3, 6 and 10 were high at the positive control group, when compared with the negative control group as expected and it was statistically significant (p=0.010, p=0,016, p=0.006). Serum levels of IL6, IL10 and TNF-α decreased at the cyclosporine A group and this was also statistically significant (p=0.006,p=0,004,p=0.010). Dexamethasone group serum level of any parameter was not statistically significant for the comparison of any other group.

Conclusions:

This study underlines the fact that; cyclosporine A is successful at decreasing the allergic mediators of the experimental model of the allergic conjunctivitis in mice. Dexamethasone treatment did not show the same effect. To the best of our knowledge this study is the first study showing the effect of topical cyclosporine A and dexamethasone on serum allergic mediator levels on an experimental model of allergic conjunctivitis. These results could be usefull while treating the patients with allergic conjunctivitis.

Financial Disclosure:

NONE

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