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Bioequivalent to the surface layers of the cornea

Poster Details

First Author: A.Kavelina UKRAINE

Co Author(s):    A. Popandopulo   O. Ivanova   G. Drozhzhina        

Abstract Details

Purpose:

Create the necessary conditions for the cultivation of the two cell cultures of corneal human on the surface amnion.

Setting:

V.K. Gusak Institute of Urgent and Recovery Surgery of the National Academy of Medical Sciences of Ukraine, laboratory cellular and tissue culture,Donetsk, Ukraine

Methods:

Used primary cultures of limbal stem cells and squamous cells isolated from human corneas kadavernyh. In the 8-well plates were placed amnion size thawed and placed 1x1sm 20000 limbal stem cells in 200 µl of medium. After reaching the monolayer placed 20000 squamous cells and cultured 3 days. Immunohistochemical staining was performed on specific markers p63, EGFR, Ki 67, TGFb, CD 117, ALDH3A1, ABCDG2, integrin β1, cytokeratin 19, cytokeratin 3/12.

Results:

Layerwise culturing of limbal stem cells and squamous cells favorably carried out on both sides of the amnion. Established cell-tissue graft is bioequivalent to the surface layers of the cornea. The results showed the expression of different antibodies, and also showed the integrity of the substrate with the cultured cells of the cornea. it can be used in the future for regeneration of the front layers of the cornea.

Conclusions:

Thus, the proposed model is an bioequivalent to the surface layers of the cornea can become an alternative to keratoplasty and is able to stimulate damaged cornea in reparative regeneration.

Financial Disclosure:

NONE

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