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The effect of anterior stromal puncture using Q-switched Nd:YAG laser on corneal wound healing

Poster Details

First Author: M.Hamdy EGYPT

Co Author(s):                        

Abstract Details


This study aimed to evaluate the impact of normal healing and the anterior stromal puncture using Q-switched Nd:YAG laser treatment of injury on corneal protein after different periods.


The energy setting of Nd: YAG laser was 0.5 mJ per pulse or less, the spot size was 10 microns; the cone angle was 16 degree; the wavelength was 1064 nm and the duration of the pulse was 4 nanosecond.


21 rabbits classified into three groups. control group, The rest of the animals, corneal epithelium injured by needle and divided into:(A) Normal healing group: divided into three subgroups and the animals left for normal healing for1 day, 1 week, and 4 weeks, (B) Laser treated group: divided the same and subjected to anterior stromal puncture using Q-switched Nd: YAG laser and the animals were left for 1 day, 1 week, and 4 weeks respectively. the corneas were isolated for estimation of total protein content, sodium dodecyl sulfate polyacrylamide gel electrophoresis, total antioxidtive capacity, total oxidative capacity and oxidative stress index.


Corneal total protein showed increment in percentage change in normal healed groups after 1 day, 1 week, 4 weeks by values 93%, 68% and 39%. Nd: YAG laser treated group showed better improvement in corneal protein with percentage changes 58%, 29%, and 7.5%. In SDS- PAGE, a protein band at 110 KD appeared in epithelium for both normal healed and Nd:YAG laser treated group with changes in the peaks. after 4 weeks the peak at 110 KD disappeared in the injured epithelium treated with Nd:YAG. After four weeks, OSI in laser treated corneas showed balance between antioxidative and oxidative capacity.


Conclusion: Anterior stromal puncture by Q-switched Nd:YAG laser is an effective, simple, safe and promising procedure to treat recurrent corneal erosion than normal healing.

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