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Effect of femtosecond laser-assisted cataract surgery on posterior capsule opasification in the human capsular bag in-vitro

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Session Details

Session Title: PCO. Cataract Surgery Complications/Management

Session Date/Time: Tuesday 08/09/2015 | 08:00-10:30

Paper Time: 08:06

Venue: Room 1

First Author: : C.Wertheimer GERMANY

Co Author(s): :    T. Kreutzer   M. Dirisamer   K. Eibl-Lindner   D. Kook   S. Priglinger   W. Mayer     

Abstract Details


To compare the rate of posterior capsule opacification i.e lens epithelial cell growth and survival in the human capsular bag model in-vitro between conventional cataract surgery using phacoemulsification (phaco) technique and femtosecond laser-assisted cataract surgery.(FLACS).


Cell- and molecular biology laboratory, Department of Ophthalmology, Ludwig Maximilians University, Munich, Germany.


For the in-vitro human capsular bag model, 18 cadaver eyes from 9 human donors underwent three types of cataract surgery. Three groups consisting of six capsular bags were established, i.e. FLACS, phaco and extracapsular cataract extraction (ECCE). The capsular bag was transferred into cell culture conditions after using one of the defined surgical approaches. Cellular growth of lens epithelial cells was observed and foto-documented. The time until full cell-coverage of the capsular bag was measured.


For this study we established a human capsular bag model by using femtosecond laser-assisted cataract surgery. There was no statistically significant difference in time until cell-coverage of the human donor capsular bag in-vitro in all three surgical settings (ECCE vs. Phaco p=0.6; ECCE vs. FLACS p=1.0; Phaco vs. FLACS p=1.0).


In our in-vitro human capsular bag model, we could not observe a statistically significant difference of PCO formation. Therefore PCO formation might not be attributed to the types of surgery. Furthermore, this study shows that FLACS can be used for the human capsular bag model preparation and suggests the human capsular bag model for future research in this area.

Financial Interest:


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