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Corneal melting after acute ultraviolet radiation exposure: an animal model

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Session Details

Session Title: Cornea
Session Date/Time: Sunday 28/02/2016 | 08:30-11:00
Paper Time: 09:42
Venue: MC2 Room
First Author: : M.Kronschläger AUSTRIA
Co Author(s): :    N. Talebizadeh   Z. Yu   S. Lofgren   O. Findl     

Abstract Details


There have been case reports of corneal melting after corneal cross-linking. The purpose of the study was to evaluate toxicity of ultraviolet radiation (UVR) in the rat cornea.


Vienna Institute for Research in Ocular Surgery, Department of Ophthalmology, Hanusch Hospital, Vienna


In vivo exposure of 16 Sprague Dawley rats to 0.56 mW/cm^2 UVR (λmax: 300 nm; λ0.5: 10 nm) for 15 min was performed. Contralateral control eyes were shielded during exposure. The animals were then separated into 4 latency groups and sacrified 1, 5, 24 and 120 h post exposure. Eye globes were taken and analysed for apoptosis with the TUNEL method. Additionally, H&E staining was used for revealing histopathological changes.


All 3 corneal layers presented apoptosis 5 h after UVR exposure with a peaking signal at 24 h. Corneal melting together with a stromal neutrophil infiltration appeared at 120 h after exposure.


In conclusion, UVB exposure causes corneal melting 5 times below the irradiance level of corneal cross-linking. Corneal melting takes place in the time window 24 to 120 h and is probably induced by neutrophils.

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