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A novel method using collagen cross-linking to evaluate the ability of the LaserACE procedure to decrease ocular rigidity as it relates to the efficiency of intraocular accommodative forces

Poster Details

First Author: A.Hipsley USA

Co Author(s):    G. Waring IV   E. Hsiao   J. Wang        

Abstract Details


To develop a novel method of collagen crosslinking of the sclera in order to mimic age-related ocular rigidity for the purpose of assessing the potential effects of a laser procedure to enhance biomechanical ocular plasticity for the benefit of intraocular accommodative resultant force efficiency.


Study was performed in a research setting Department of Biomedical Engineering, National Taiwan University.


50 freshly harvested porcine eyes were separated into 4 groups: Group A) plain eyes(simulate normal young eyes); Group B) X-linked eyes with different degree of cross-linkage (simulate aging eyes of various degrees); Group C) X-linked then ablated eyes( simulate aging eyes treated with LaserACE procedure); Group D) Ablated eyes without X-link(young control eyes).LaserACE pattern(9 spot matrix) in Groups C & D were performed with a 2.94um Er:Yag laser. A custom made measurement System including a pressure transducer (up to 5psi), a dosage injector controller , a data computerized reader and tissue holding frame. Each porcine eye was fixed to the frame while distilled water was injected into the vitreous chamber at a constant flow rate of 8.42 ul/sec. Pressure was plotted vs injected volume curve to estimate ocular rigidity changes from the slope of the linear regression line.


The Collagen X-linking effectively represented various distinct ocular rigidity markers over time. The groups ablated with the LaserACE pattern showed a statistically significant decrease in rigidity. The Slope of P-V curvature also showed a linear regression correlation at high pressure end with y=1.0697x-4.6587 ; R2=0.9939.


The porcine scleral globe response to the cross-linking successfully characterized the ocular rigidity that occurs at various points of age. The early response of the porcine sclera to the LaserACE ablation pattern with an Er:Yag correlates positively with a decrease in ocular rigidity. An improvement of ocular plasticity may be beneficial to decrease the mechanical resistance of the ocular wall to improve the resultant centripetal forces of mechanical accommodation. Collagen X-linking may be a novel tool to evaluate the influence of ocular rigidity on the globe. Likewise, it may also serve as a method to determine mechanism of action of the LaserACE procedure and its impact on accommodative resultant force efficiency

Financial Disclosure:

... receives consulting fees, retainer, or contract payments from a company producing, developing or supplying the product or procedure presented, ... travel has been funded, fully or partially, by a company producing, developing or supplying the product or procedure presented, ... research is funded, fully or partially, by a company producing, developing or supplying the product or procedure presented

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