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First Author: S.Chiang TAIWAN
Co Author(s): D. Lu M. Tsai
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Ankylosing spondylitis (AS) is an autoimmune diseases characterized by chronic systemic inflammatory disorder of the axial skeleton and shows significant inherited susceptibility. About 90% of the patients express the HLA- B27 genotype. Although great progress has been made recently in exploring the etiology and pathogenesis of AS, its macular pathological mechanism remains to be further defined. Our study aim to detect candidate AS-associated serum proteins by comparative proteomics to provide research clues to early diagnosis and treatment of AS.
Department of Ophthalmology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
To better understand the pathophysiology of AS and to identify AS with uveitis-associated risk factors, a gel-based proteomics analysis was performed to compare serum protein profiles of AS patients with HLA-B27 positive, HLA-B27 negative and healthy cases(controls). MALDI-TOF MS was then performed to identify protein spots that were differentially expressed between these three groups and western blot analysis was used to validate the expressional change of protein demonstrated by proteomics.
Six proteins were found to vary in relation to AS groups compared with control samples. A group of four highly expressed protein spots was observed in AS patients" profiles with HLA-B27 positive, they were derived from superoxide dismutase, proteasome subunit ? type 3, proteasome activator complex subunit ?, and keratin.
The protein composition in serum was significantly different between both groups (disease and control). The identified proteins might be candidate AS-associated proteins, promising diagnosis indicators or therapeutic targets for AS, and also may contribute to some of the clinical manifestations of this disease.