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Microbial and fungal sampling and culture evaluation of an ambulatory ophthalmological unit: a novel protocol

Poster Details

First Author: G.Chatzilaou GREECE

Co Author(s):    A. Kanellopoulos   G. Asimellis   C. Sophatzidi        

Abstract Details


To evaluate an elaborative, novel protocol of surface and air sampling of the several levels of sterility operating rooms in a modern ophthalmological ambulatory surgical center

Setting: Eye Institute, Athens, Greece


Methods Of the 8 rooms comprising the ambulatory surgical center 3 levels of sterility were defined: Level 1: changing rooms and pre-op and recovery area, Level 2 connecting hallways and sterilization room, Level 3: intraocular and laser refractive operating rooms. All rooms were sampled bi-weekly with swab smears of the floors, walls and ceilings as well as by sampling the air with a specialized device (Biotest handheld RCSR air sampler). Last the air filters of the ventilation system were removed, swabbed and cultured as well. All cultures were analyzed and processed in a specialized microbiology laboratory.


Level 1 areas showed air samples: 200 CFU/m3 (microbial only colonies per cubic meter) wall and door swabs: 5 CFU of bacteria only (no fungus) Level 2 areas showed: air samples: 120 CFU/m3, wall, door and microscope swabs: 2 CFU, bacteria only no fungus Level 3 areas showed: air samples: 88 CFU/m3 wall, door and microscope swabs: 3 CFU, bacteria only (no fungus). Almost all bacteria were Staphylococcus epidermidis with one CFU of Staphylococcus lugdunensis


This novel elaborative microbial monitoring system of an ambulatory operating unit provided detailed data of not only the classification and population of microbes, but the exact topographic location in the operating rooms. This information caution operating room personnel on ongoing disinfecting techniques and methods, as well as surgeons in planning appropriate antimicrobial prophylaxis for prospective patients as well as a topographic map of the most and least disinfected spots in the operating room

Financial Disclosure:


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