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Analysis of histomorphological changes after corneal collagen cross-linking in the treatment of progressive keratoconus

Poster Details

First Author: O.Shunkevich RUSSIA

Co Author(s):    E. Solodkova   I. Melikhova   V. Fokin        

Abstract Details


to study histomorphological changes, occurring in cornea after corneal collagen crosslinking in treatment of progressive keratoconus according to findings of confocal microscopy and optical coherent tomography (OCT).


The S.N. Fyodorov Eye Microsurgery Federal State Institution, Volgograd Branch


16 patients (16 eyes) with keratoconus of I-II degree, aged from 18 to 30 years were examined. Prior to and after the surgery (at checkpoints of 1,3,6 and 12 months) an extensive ophthalmological examination, including OCT of the cornea (OCT RS-3000, Nidek, Japan), confocal microscopy with count of endothelial cells density (Confoscan4, Nidek, Japan) was performed. When treating according to a modified technique depth-metered deepithelialization was made on excimer laser SCHWIND Amaris-500 Hz, Germany, equipped with a function of intrasurgical on-line pachymetry. Exposition to ultraviolet A was made by means of ḋUV-XṠ device (IROC Inc., Switzerland).


ophthalmobiomicroscopy, performed 1 month postoperatively, revealed emergence of linear opacification in medium and posterior stromal layers – a so-called “demarcation line” or “posterior stromal haze”. Depth of its formation averaged 260ḟ35 ṁm. It tended to shift towards surface with time: 3 months postoperatively it was observed at the depth of 248ḟ20 ṁm, 6 months postoperatively – at the depth of 240ḟ10 ṁm and 12 months postop. - at the depths of 235ḟ10 ṁm. Optical zone reinnervation completed by check-up examination 6 months postoperatively.


Analysis of histomorphological results of corneal collagen crosslinking, evaluated basing on OCT-data and findings of confocal microscopy, revealed that certain specific changes could be observed within medium and deep stromal tissues, either in a form of demarcation line, which tends to vary with time in its density and localization, or as repopulation of keratocytes with intensified production of collagen, epithelial hyperplasia and stromal compaction.

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