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Descemet stripping with automated endothelial keratoplasty: time-domain anterior segment OCT and in vivo confocal analysis

Poster Details

First Author: R.Mencucci ITALY

Co Author(s):    I. Paladini   R. Tartaro   E. Favuzza   U. Menchini     

Abstract Details


To evaluate the in vivo corneal changes in patients with Fuchs dystrophy before and after Descemet Stripping with Automated Endothelial Keratoplasty (DSAEK) using confocal microscopy and time-domain anterior segment OCT


University of Florence


We analysed 54 patients (17 men, 37 women; mean age 73.8 years) with Fuchs dystrophy who underwent DSAEK. Confocal microscopy and time-domain anterior segment OCT were performed before and one, three, six and twelve months after DSAEK. We evaluated subepithelial reflectivity, donor recipient interface haze, interface particles, endothelial cell count and thickness of the graft.


Preoperatively, corneal epithelial edema, subepithelial reflectivity, endothelial count were evaluated in all patients. After DSAEK, subepithelial haze, donor-recipient interface haze and depth, interface particles and endothelial count were observed ; postoperative haze and particles decreased statistically significantly (P < .05) over follow-up.The depth of the recipient donor interface was statistically similar between the two instruments. Interface and subepithelial reflectivity were significantly correlated with visual acuity (P?

In vivo confocal microscopy and time-domain anterior segment OCT can recognize subclinical corneal abnormalities present at different degree after DSAEK. Further studies in a larger number of patients and long-term follow-up using this device are necessary to fully understand long-term corneal stromal changes after DSAEK.

Financial Disclosure:


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