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First Author: L.Ibares Frías SPAIN
Co Author(s): P. Gallego R. Cantalapiedra J. Merayo-Lloves M. Martínez-García
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To evaluate the reversibility of the clinical and histological changes induced in the corneas of an animal model after removing intracorneal ring segment (ICRS) at different time points.
University of Valladolid
We performed surgery for this study in 24 eyes of an experimental animal model (Gallus Domesticus ) of ICRS surgery (Ferrara technique) previously described by us. The animals were randomized in two groups, in all of them, we implanted one segment in each eye and was removed in different times, in group 1 at one month after implantation and group 2 at three months after implantation. In each Group corneas were processed at different time points for clinical and histological analysis, 7 days, 15 days, 1 month, 2 months , and 3 months. For histological analysis hematoxilin eosine and Masson Trichromic stains were made. Trasmissión electrón mycroscopy of the samples were made too. Clinical evaluation was made with a surgical microscope.
Explantation was performed correctly in all cases, no complications were found. Histological analysis:At 7 days we observed hipercelularity and abnormal conformation of the collagen fibres, which at 15 days change to normal, with persistente of the hipercelularity.Later, the hipercelularity decreased to normal values similar to preoperative at 2 months in group 1 and at 4 months in group 2. Minimal changes could be observed in electron mycroscopy up to the end of the study. As regards clinical evaluation:in the first weeks we observed deposits of cells and a moderate degree of corneal opacity( haze) around the space where the ICRS was implanted, signs which decreased progressively. At 3 months no deposits of cells were found, only a leucoma in the place of the segment.
ICRS are safety explanted from corneas of an experimental animal model.The reversibility of the clinical and histological findings depended on the time explanted. This results must be understood when another surgery is planned after explantation of intrastromal ring segments.