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First Author: M.Mohammadpour IRAN
Co Author(s): M. Jabbarvand E. Delrish
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Intravitreal injection of silicate nanoparticles has been safely used for management of choroidal neovascularization. However, topical application of silicate particles in nano scale was not used before. This study was performed to investigate penetration and transmission of topical silicate nanoparticles (SiNps) through the corneal stroma.
Farabi Eye Hospital,Tehran University of Medical Sciences
The permeability of 5 to 50 nm nanoparticles over 6 hours was examined across isolated corneal buttons. In vitro experiments were performed using human, sheep, and rat corneal buttons. To visualize the transport and diffusion of nanoparticles through the tissue, different titrated sizes of SiNPs were applied on the corneal surface and evaluated at 30 minutes and 6 hours after SiNPs loading by scanning electron microscope (SEM). After performing the transport across the corneal tissues as described above, the part of the tissue exposed to the nanoparticle suspension was cut off and embedded in an optimal cutting temperature medium for frozen sectioning. Sections with 10 ṁm thickness were cut and visualized using SEM.
Transmission of SiNPs (5-50 nm) through the corneal stroma was shown successfully with electron microscopy images. The penetration amount of SiNPs was directly related to the size of SiNPs .The smaller size showed more rapid penetration through the corneal stroma.
Topical administration of SiNPs, as a non-invasive, inexpensive and available modality, with acceptable penetration through the corneal stroma and deep to the ocular tissues, may be a suitable alternative for invasive intravitreal injection of other expensive anti-VEGF agents. Further studies can be performed on efficacy of transmission of SiNPs through the corneal barriers to manage corneal and choroidal neovascularization.