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A 5 year report of in vitro antimicrobial susceptibility of bacterial keratitis isolates and normal ocular commensals
Session Title: Inflammation
Session Date/Time: Tuesday 08/10/2013 | 16:30-18:00
Paper Time: 17:02
Venue: Auditorium (First Floor)
First Author: : S.Saha INDIA
Co Author(s): : J. Sengupta A. Khetan
To report the antibiotic susceptibility profile of bacterial keratitis isolates and commensal microbial flora of conjunctival cul de sac isolated over the last 5years(2008-2012).
Tertiary care eye hospital of eastern India
Laboratory records of all consecutive cases of bacterial corneal ulcers and normal commensals were retrospectively reviewed. Data noted included microorganism isolated and antibiotic culture sensitivity to the fourth generation fluoroquinolones (moxifloxacin, gatifloxacin),fluoroquinolones (ofloxacin and ciprofloxacin), cefazolin, and to the aminoglycosides (tobramycin and gentamicin) was performed using by the Kirby-Bauer disc diffusion method.
A total of 410 bacterial isolates were identified from corneal ulcer (268 cases), postoperative endophthalmitis (36 cases) and conjunctival swab culture (107 cases). Of these, 315 (76.82%) were Gram-positive and 95 (23.17%) were Gram-negative. Staphylococcus epidermidis (197, 48.04%) was the most common organism followed by Staphylococcus aureus (78, 19.02%) among gram positive and Pseudomonas was the commonest (90, 22%) among gram negative bacteria.Lowest MIC was noted for moxifloxacin (0.08 µg/ml) and cefazolin (0.1µg/ml) in Staphylococcus group, Ciprofloxacin (0.008µg/ml) and Gatifloxacin (0.016 µg/ml) in Gram negative bacilli.3 Gram positive bacteria (Staph aureus 2 cases and Staph epidermidis 1 case) and 4 Gram negative bacteria (all are Pseudomonas sp) isolated from postoperative endophthalmitis were found resistant to all mentioned antibiotics.
Incidence of drug resistance is uncommon amongst the bacterial isolates obtained from infective keratitis and commensals in conjunctival cul de sac. However, resistant strains are more common in isolates obtained from cases of postoperative endophthalmitis.