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Vital dyes increase lens capsule stiffness

Session Details

Session Title: New evaluation tools in refractive surgery

Session Date/Time: Sunday 06/10/2013 | 08:00-09:30

Paper Time: 08:21

Venue: Forum (Ground Floor)

First Author: : A.Wolf GERMANY

Co Author(s): :    M. Benoit   R. Schumann   S. Mauell   P. Henrich   M. Kernt   C. Haritoglou

Abstract Details


To assess potential changes of the mechanical properties of the lens capsule following staining with brilliant blue (BB), indocyanine green (ICG) and trypan blue (VB).


Experimental study, Department of Ophthalmology and Applied Physics and Center for NanoScience, Ludwig-Maximilians-University, Munich.


Fifteen unstained lens capsules were obtained during uneventful cataract surgery. Sepcimens were dissected into seven wedge shaped parts. Three fragments were stained with 0.025% BB (Brilliant Peel®; Fluoron GmbH, Germany), 0.05% ICG (Pulsion, Germany) and 0.06% VB (vision blue ® DORC, Netherlands) respectively for one minute, another three specimens additionally illuminated using a standard light source. The seventh fragment served as an untreated control. All specimens were analysed using atomic force microscopy (AFM) in contact mode with a scan rate of 0.6 Hz. Two scan regions of 10×10?m were chosen and stiffness was determined by using AFM in a force spectroscopy mode. The force curves were performed with a data rate of 5000 Hz.


Staining of the samples resulted in an increase of tissue stiffness (BBG: p<0.001; ICG: p<0.01; VB: p<0.05). Additional illumination (indicated by "L") after staining did further increase the stiffness of the tissue but not significantly. Relative elasticity values were measured 1.61±0.15 for BB, 2.04±0.21 for BBL, 1.63±0.22 for ICG, 2.01±0.22 for ICGL, 1.23±0.11 for VB and 1.39±0.11 for VBL. In relation to unstained tissue the relative elasticity of the stained tissue increased by 1.2 fold following after illumination


Staining significantly increases the mechanical properties of the human lens capsule

Financial Interest:


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