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Corneal endothelial reaction on He-Ne 0.63 µm laser impact in cell culture

Poster Details

First Author: S.Kopayev RUSSIA

Co Author(s):    S. Borzenok   V. Kopayeva   O. Kravchuk        

Abstract Details


to determine in experiment biological impact of He-Ne 0.63 µm laser which accompanies high energy Nd-YAG 1.44 µm radiation in laser cataract extraction surgery.


Fyodorov Eye Microsurgery State Institution, Moscow


after performing laser cataract extraction (LCE) surgery on 12 cadaver eyes with use of two laser guided on single optical fibre - endodissector Nd-YAG 1.44 µm and boistimulator He-Ne 0.63 µm - corneal endothelium cell sheets were separated. Control sheets were separated from paired eyes which were not operated. Survival cell cultures were subjected to radiation of He-Ne laser 0.63 µm in different trays with 3 and 10 min. exposure later incubated till 21 days with every day automatic control.


compared to control cultures survival rates of corneal endothelium 7 days after irradiation were 2 times higher; in 21 days - 2.7 times higher (p≤0.005). This proves antiapoptosis system activation with simultaneous absence of phototoxic effect.


He-Ne laser 0.63 µm during laser cataract extraction exerts endoocular biostimulating effect on corneal endothelial cells. No phototoxic effect found. FINANCIAL INTEREST: NONE

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