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Corneal endothelial reaction on He-Ne 0.63 µm laser impact in cell culture

Poster Details

First Author: S.Kopayev RUSSIA

Co Author(s):    S. Borzenok   V. Kopayeva   O. Kravchuk        

Abstract Details



Purpose:

to determine in experiment biological impact of He-Ne 0.63 µm laser which accompanies high energy Nd-YAG 1.44 µm radiation in laser cataract extraction surgery.

Setting:

Fyodorov Eye Microsurgery State Institution, Moscow

Methods:

after performing laser cataract extraction (LCE) surgery on 12 cadaver eyes with use of two laser guided on single optical fibre - endodissector Nd-YAG 1.44 µm and boistimulator He-Ne 0.63 µm - corneal endothelium cell sheets were separated. Control sheets were separated from paired eyes which were not operated. Survival cell cultures were subjected to radiation of He-Ne laser 0.63 µm in different trays with 3 and 10 min. exposure later incubated till 21 days with every day automatic control.

Results:

compared to control cultures survival rates of corneal endothelium 7 days after irradiation were 2 times higher; in 21 days - 2.7 times higher (p≤0.005). This proves antiapoptosis system activation with simultaneous absence of phototoxic effect.

Conclusions:

He-Ne laser 0.63 µm during laser cataract extraction exerts endoocular biostimulating effect on corneal endothelial cells. No phototoxic effect found. FINANCIAL INTEREST: NONE

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