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Visualization of microbial biofilms on intraocular lenses by scanning electron microscopy

Poster Details

First Author: S.Kivanc TURKEY

Co Author(s):    V. Kılıç   G. Gullulu   M. Kivanc        

Abstract Details



Purpose:

To investigate biofilm production on lenses from different companies.

Setting:

Anadolu University, Faculty of Science

Methods:

The icaA , icaD and bap positive S. epidermidis KA 15.8 and icaA , icaD and bap negative S. epidermidis KA 14.5 were used. Lenses were contaminated with S. epidermidis solutions containing 107 colony-forming units. IOLs were inoculated into plate containing 0.25% glucose tryptic soy broth and incubated 24h at 37C. Biofilms on intraocular lenses of different companies were also examined by scanning electron microscopy (SEM). After lenses were taken away from bacteria solution; they were washed 3 times for removing non-adhesive bacteria. After that bacteria were fixed in 2.5 % glutaraldehyde in 0.1 M cacodylate buffer for 2 hours at room temperature, postfixed in 1% OsO4 for 1 hour, and dehydrated in a series of ethyl alcohol (30, 50, 70, 90 and 100 %), each for 15 minutes. Samples were incubated in 100 % ethanol two times for 20 minutes. After critical point - dried using CO2 and sputter-coated with gold according to standard procedures, specimens were investigated with a Zeiss Ultra 50 SEM operated at 5 kV accelerating tension.

Results:

Intraocular lens technologies are different in different companies and we noticed that this difference is consistent with biofilm formation.

Conclusions:

The amount of biofilm on the lens, vary from company to company. FINANCIAL INTEREST: NONE

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