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Microbiological profile and antibiotic susceptibility of bacterial keratitis: a 10 year review

Poster Details

First Author: C.Hsiao TAIWAN

Co Author(s):                  

Abstract Details


To review the distribution, current trend and antibiotic susceptibility patterns of bacterial keratitis isolated in Chang Gung Memorial Hospital over the past 10 years.


Chang Gung Memorial Hospital


We retrieved the computer database of suspected bacterial keratitis cases from the microbiology laboratory in our hospital between January 1, 2003 and December 31, 2012, then analyzed culture results as well as in vitro antibiotic susceptibility. Linear by linear association was used to test for trend.


A total of 2004 corneal scrapings were taken. Culture was positive in 989 samples (49.4%), growing 1268 organisms. Bacterial keratitis accounted for 1039 isolates (81.9%), of which 533 (42.0%) were gram-positive. There was a general increase in the percentage of gram-positive isolates with time (P=.008). The most common isolate overall was Pseudomonas aeruginosa and the most common Gram- positive isolate was coagulase-negative Staphylococcus species. There was a trend toward increasing laboratory susceptibility to methicillin and clindamycin in Gram-positive bacteria (P= .001&.009). There was 93.7% and 98.8% susceptibility to ciprofloxacin in Gram-negative bacteria and P. aeruginosa.


There was a significant increase in the percentage of Gram-positive microorganisms over time. P. aeruginosa was the most isolated pathogen, and had >95% response to tested antibiotics, this was not the case for Gram-positive isolates, in which resistance to the antibiotics was more common. FINANCIAL INTEREST: NONE

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