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A new in vitro method to evaluate the microbial killing rate in CXL

Poster Details

First Author: DavidTabibian SWITZERLAND

Co Author(s):    Olivier Richoz   Farhad Hafezi           

Abstract Details


To present a new in vitro method to evaluate the killing rate of bacteria, fungus and amibia during corneal collagen cross-linking (CXL).


: Analyzing the effective killing rate of microorganisms in the cornea during CXL can be challenging, due to a depth-dependent decline of energy levels that follows the Lambert-Beer law.


We tested two types of polymers with the following characteristics: almost 100% transmission at 365nm, water content more than 50%, thickness of 300 µm. One is a chemical polymer derived from a non-toxic plastic, whereas the second has a structure similar to the human cornea. A water column of 300 µm thickness in the absence of a polymer served as control.


Both polymers showed an almost 100% microbial killing rate, whereas the killing rate in the control group was at 20%.


For UV-transmittant media, the main factor determining the microbial killing rate is polymer thickness rather than its structure. FINANCIAL INTEREST: NONE

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