Copenhagen 2016 Registration Programme Exhibitor Information Virtual Exhibition Satellite Meetings Glaucoma Day 2016 Hotel Star Alliance

10 - 14 Sept. 2016, Bella Center, Copenhagen, Denmark

This Meeting has been awarded 27 CME credits


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Deep layers morphology study in human corneas by the use of confocal microscopy

Poster Details

First Author: L. Menabuoni ITALY

Co Author(s):    A. Malandrini   A. Canovetti   L. Buzzonetti   F. Tatini   G. Magni   F. Rossi     

Abstract Details


To investigate the morphology of predescemetic collagen layers in human corneas, in order to find correlation with age, pathology and to find out new indications to surgical manoeuvres.


Nuovo Ospedale S. Stefano, Prato, Italy; Ospedale Pediatrico Bambino Gesù, Rome, Italy


Corneas from patients undergoing transplantation and healthy corneas not suitable for transplantation were collected. The samples were grouped into two: one group is from 20 adult subjects aged 59 ± 18 y.o., and the other group is from 15 young subjects, aged 12±5 y.o. The analysis of the deep stroma was performed by confocal microscopy: the corneas were sectioned and incubated with Collagen VI primary antibody, Alexa Fluor-conjugated secondary antibody, and with Phalloidin-Atto 550. Slices were mounted with fluoroshield containing DAPI and visualized using a confocal Leica SP8 scanning microscope. Images were acquired in z-series scans at 0.3 µm intervals.


Confocal microscopy analysis of posterior cornea has been performed in order to investigate relevant parameters such as cellular distribution and morphology, and collagen VI characteristics. A predescemetic layer with a higher concentration of collagen I and some samples with a higher concentration of collagen VI was found. High variability due to age and pathology was found.


It seems that there is a different distribution of collagen type I and collagen type VI in the deep layers of the cornea, depending on age and pathology. A larger study is needed to improve statistics.

Financial Disclosure:


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