Copenhagen 2016 Registration Programme Exhibitor Information Virtual Exhibition Satellite Meetings Glaucoma Day 2016 Hotel Star Alliance

10 - 14 Sept. 2016, Bella Center, Copenhagen, Denmark

This Meeting has been awarded 27 CME credits


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The effect of sulfated glycosaminoglycans (sGAG) on human corneal epithelial cell proliferation

Poster Details

First Author: G. Usanova RUSSIAN FEDERATION

Co Author(s):    A. Tereshenko   Y. Belyy   E. Takhchidi   S. Novikov           

Abstract Details


To investigate the proliferation and cytotoxic effect of different concentration of sGAG on human corneal epithelial cells (HCECs)


The S. Fyodorov Eye Microsurgery State Institution, Moscow, Russian Federation


Corneal epithelial cell cultured in DMEM/F12 supplemented with 10% fetal bovine serum (FBS), HyClone III, L-glutamine (2,5 мМ) and antibiotics (penicillin steptomitsin ). In 1st group was exposed to 0,1% sGAG , in 2nd group –0,5% sGAG, in 3d group –1% sGAG, in control group- without treatment. For the real-time monitoring of cell growth and death we used the xCELLigence real-time cell analysis system . Proliferation effect was measured as the relative impedance change -cell index (CI), across microelectronic sensors integrated into the bottom side of the membrane


After 1 hour of treatment the CI values in 1st group (0,1% sGAG) reached of 1,33± 0,10 CI , In 2nd group (0,5% sGAG) 1,33± 0,16 CI , In 3d group (1% sGAG) 1,22± 0,13 CI , in control group- 1,55±0,02 CI. After 2 hour of treatment the cell index (CI) values in 1st group reached of 2,38± 0,06 CI, In 2nd group 2,64± 0,26 CI, In 3d group- 2,55± 0,09 CI, in control group - 2,16±0,01 CI. After 3 hour of treatment the cell index (CI) values in 1st group (0,1% sGAG) reached of 2,54± 0,21 CI, In 2nd group (0,5% sGAG) 2,65± 0,24 CI, In 3d group (1% sGAG) 2,86 ± 0,11 CI, in control group- 2,37±0,02 CI


In the groups cultured with sGAG at concentrations 0,1-1% cell index was significantly higher than that in the control group. sGAG in concentration 0,1-1% stimulates corneal epithelial cell proliferation

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