Copenhagen 2016 Registration Programme Exhibitor Information Virtual Exhibition Satellite Meetings Glaucoma Day 2016 Hotel Star Alliance
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10 - 14 Sept. 2016, Bella Center, Copenhagen, Denmark

This Meeting has been awarded 27 CME credits

 

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The comparison of amniotic membrane implantation and cross-linking treatment effects in experimental acute alkali burn model with proteomic analysis

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Session Details

Session Title: Presented Poster Session: Solutions for Traumatic Cases

Session Date/Time: Tuesday 13/09/2016 | 09:30-11:00

Paper Time: 10:10

Venue: Poster Village: Pod 3

First Author: : S.Subasi TURKEY

Co Author(s): :    O. Altintas   M. Kasap   N. Guzel   G. Akpinar   S. Karaman  

Abstract Details

Purpose:

In this study, by using a two dimensional electrophoresis-based experimental approach, we aimed at understanding the nature of alkali burns and the underlying mechanisms. A secondary aim was to compare the effects of Cross-linking (CXL) and Amnion membrane transplantation (AMT) on corneal protein compositions during recovery after acute alkali burn.

Setting:

Experimental animal study Kocaeli University Department of Ophthalmology

Methods:

The right corneas of 32 rabbits were burned using a 1N solution of NaOH. Groups were formed based on the threapy applied; Group 1 (normal corneas), group 2 (non-treated control), group 3 (CXL), group 4 (AMT), group 5 (CXL+AMT). In addition to the threapies applied, a conventional medical treatment was applied to all groups including the control group. Left eyes were used as normal cornea (Group 1). The corneas were excised at day 21 and a comparative proteomic analysis was performed using 2D gel electrophoresis coupled with MALDI-TOF/TOF.

Results:

The eight proteins whose abundance changed among the groups were Keratin type 2 cytoskeletal 1 and 2 , Glyceraldehyde 3-phosphate dehydrogenase(GAPDH), SH3 domain, PHF finger protein(PHF), Collagen 6, S100 A11 and Transketolase(TKT). The regulation trends were calculated in two different ways; (1) over the healthy cornea (/G1)and (2) over the control group (/G2). There was a decrease in PHF levels in all groups while increases were observed in SH3 Domain Binding Protein and Protein S100-A11 when comparisons were made over G1. When regulation ration over G2 were examined, S100-A11 and TKT displayed increases in their abundance while keratines displayed decreases.

Conclusions:

Ingenuity Pathway analysis predicted that the Keratinocyte Pathway was appeared to be affected by the applied treatments. A nonoxidative branch of Pentose Phosphate pathway also appeared to be affected from the alkali burn.

Financial Disclosure:

NONE

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