Copenhagen 2016 Registration Programme Exhibitor Information Virtual Exhibition Satellite Meetings Glaucoma Day 2016 Hotel Star Alliance

10 - 14 Sept. 2016, Bella Center, Copenhagen, Denmark

This Meeting has been awarded 27 CME credits


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The comparison of amniotic membrane implantation and cross-linking treatment effects in experimental acute alkali burn model with proteomic analysis

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Session Details

Session Title: Presented Poster Session: Solutions for Traumatic Cases

Session Date/Time: Tuesday 13/09/2016 | 09:30-11:00

Paper Time: 10:10

Venue: Poster Village: Pod 3

First Author: : S.Subasi TURKEY

Co Author(s): :    O. Altintas   M. Kasap   N. Guzel   G. Akpinar   S. Karaman  

Abstract Details


In this study, by using a two dimensional electrophoresis-based experimental approach, we aimed at understanding the nature of alkali burns and the underlying mechanisms. A secondary aim was to compare the effects of Cross-linking (CXL) and Amnion membrane transplantation (AMT) on corneal protein compositions during recovery after acute alkali burn.


Experimental animal study Kocaeli University Department of Ophthalmology


The right corneas of 32 rabbits were burned using a 1N solution of NaOH. Groups were formed based on the threapy applied; Group 1 (normal corneas), group 2 (non-treated control), group 3 (CXL), group 4 (AMT), group 5 (CXL+AMT). In addition to the threapies applied, a conventional medical treatment was applied to all groups including the control group. Left eyes were used as normal cornea (Group 1). The corneas were excised at day 21 and a comparative proteomic analysis was performed using 2D gel electrophoresis coupled with MALDI-TOF/TOF.


The eight proteins whose abundance changed among the groups were Keratin type 2 cytoskeletal 1 and 2 , Glyceraldehyde 3-phosphate dehydrogenase(GAPDH), SH3 domain, PHF finger protein(PHF), Collagen 6, S100 A11 and Transketolase(TKT). The regulation trends were calculated in two different ways; (1) over the healthy cornea (/G1)and (2) over the control group (/G2). There was a decrease in PHF levels in all groups while increases were observed in SH3 Domain Binding Protein and Protein S100-A11 when comparisons were made over G1. When regulation ration over G2 were examined, S100-A11 and TKT displayed increases in their abundance while keratines displayed decreases.


Ingenuity Pathway analysis predicted that the Keratinocyte Pathway was appeared to be affected by the applied treatments. A nonoxidative branch of Pentose Phosphate pathway also appeared to be affected from the alkali burn.

Financial Disclosure:


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