Copenhagen 2016 Registration Programme Exhibitor Information Virtual Exhibition Satellite Meetings Glaucoma Day 2016 Hotel Star Alliance
title

10 - 14 Sept. 2016, Bella Center, Copenhagen, Denmark

This Meeting has been awarded 27 CME credits

 

escrs app advert yo advert

In vitro evaluation of the amebicidal activity of Foeniculum vulgare (fennel) seed essential oil against trophozoites and cysts of Acanthamoeba spp related Acanthamoeba keratitis

Search Title by author or title

Session Details

Session Title: Presented Poster Session: Endophthalmitis/Ocular Infections

Session Date/Time: Monday 12/09/2016 | 15:00-16:30

Paper Time: 15:50

Venue: Poster Village: Pod 3

First Author: : F.Khoshzaban IRAN

Co Author(s): :    F. Ghaffarifar   T. Nayeri   M. Jabarvand Behrooz        

Abstract Details

Purpose:

The aim of this study was to evaluate the in vitro amoebicidal activity of Foeniculum vulgare (fennel) seed essential oil (EO) on 8 isolates of Acanthamoeba from Acanthamoeba keratitis patients.

Setting:

In the present study, isolate of Acanthamoeba was separated from patient with keratitis. The isolates were cultured in NNA. Eight samples of Acanthamoeba were collected. DNA extraction and amplification of 18s rRNA gene by PCR successfully were conducted.

Methods:

In the next step in the presence of fennel (EO) (ranging from 1.25 to 10.0 mg/ml), numbers of the viable Acanthamoeba trophozoites and cysts were determined during the experimental process (72 hour).The viability assays 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) was tested for their suitability as time-saving alternatives to the classical manual or direct-counting method, assessing the effect of the fennel (EO).

Results:

Numbers of the viable Acanthamoeba trophozoites and cysts were decreased (72 hour).

Conclusions:

MTT assay indicated that the viability assays were isolate-dependent leading in some cases to an overestimation of the real situation of viable cells. .

Financial Disclosure:

NONE

Back to previous