Copenhagen 2016 Registration Programme Exhibitor Information Virtual Exhibition Satellite Meetings Glaucoma Day 2016 Hotel Star Alliance

10 - 14 Sept. 2016, Bella Center, Copenhagen, Denmark

This Meeting has been awarded 27 CME credits


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Microbial analysis and disinfectant sensitivity on worn cosmetic contact lenses

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Session Details

Session Title: Presented Poster Session: Endophthalmitis/Ocular Infections

Session Date/Time: Monday 12/09/2016 | 15:00-16:30

Paper Time: 15:00

Venue: Poster Village: Pod 3

First Author: : E.Shen TAIWAN

Co Author(s): :    F. Hu              

Abstract Details


To analyze the attached microorganisms found on various worn cosmetic contact lens (CL) materials and compare the differences in sensitivity to common disinfectant solutions


National Taiwan University Hospital


20-35 year old healthy volunteers with signed informed consent were recruited. Subjects wore nelfilcon,hilafilcon,or etafilcon daily disposable CL for 8 hours/day for 2 weeks. The lenses are collected aseptically and cut in half. One half of the lens is macerated and attached microbes identified. The other half is used for a 2 hour incubation with 10^8 cfu/ml of P. aeruginosa (cytotoxic strain PA103 or invasive strain PAK). The lenses are then macerated and serial dilution performed to enumerate the number of viable bacteria. P. aeruginosa incubated lenses were soaked with various disinfectants. The bacteria remaining on CL is compared.


The most commonly identified microbes attached to non-symptomatic lens wearing volunteers were coagulase negative Staphylococcus, P. aeruginosa, and Streptococci. Adhesion of the cytotoxic strain PA103 was found to be greatest for hilfilcon lenses (34.8x104cfu/ml) compared to etafilcon (33.1 x104cfu/ml) and nelfilcon (15.5x104cfu/ml) (P<0.05; ANOVA). Similar trend is noted for adhesion of the invasive strain PAK: hilfilcon (30.9x104 cfu/ml)>etafilcon(37.5x104 cfu/ml) >nelfilcon(10.1x104 cfu/ml) (P<0.05; ANOVA). At 100% suggested time, all tested disinfectants were able to kill all bacteria. However, at less than 75% of suggested disinfection time, significant number of viable cytotoxic P. aeruginosa remained (P=0.03, t-test).


P. aeruginosa, a commonly identified pathogen in CL-associated microbial keratitis, is frequently found attached to CL of asymptomatic individuals. Cytotoxic strains are more resistant to MPS solution especially Renu

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